Skin care formulations

ABSTRACT

Disclosed is a method of firming skin or reducing the appearance of fine lines or wrinkles comprising topically applying, to skin in need thereof, a composition comprising palmitoyl tetrapeptide 7 and a dermatologically acceptable vehicle comprising water, glycerin, butylene glycol, propylene glycol, and a chelating agent, wherein topical application of the composition to the skin firms the skin or reduces the appearance of fine lines or wrinkles, and wherein the composition is not a sunless tanning composition.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No.14/223,729 filed Mar. 24, 2014, which is a continuation of U.S.application Ser. No. 13/237,573 (now U.S. Pat. No. 8,691,300), filedSep. 20, 2011, which is a continuation of U.S. application Ser. No.12/871,557 (now U.S. Pat. No. 8,048,456), filed Aug. 30, 2010, whichclaims the benefit of U.S. Provisional Application No. 61/238,001, filedAug. 28, 2009. The contents of these applications are incorporated byreference.

BACKGROUND OF THE INVENTION

A. Field of the Invention

The present invention relates generally to compositions that can be usedto improve the skin's visual appearance. In one particular aspect, thecomposition includes a combination of palmitoyl tetrapeptide-7,methylsilanol mannuronate, Lactobacillus ferment, and plant extractsfrom Punica granatum, Castanea sativa, Gossypium hirsutum, and Euterpeoleracea. However, other skin active ingredients and formulations andcombinations are contemplated throughout this specification.

B. Description of Related Art

With ageing, chronic exposure to adverse environmental factors, ormalnutrition, the visual appearance, physical properties, andphysiological functions of skin can change in ways that are consideredvisually undesirable. The most notable and obvious changes include thedevelopment of fine lines and wrinkles, loss of elasticity, increasedsagging, loss of firmness, loss of color evenness or tone, coarsesurface texture, and mottled pigmentation. Less obvious, but measurablechanges which occur as skin ages or endures chronic environmental insultinclude a general reduction in cellular and tissue vitality, reductionin cell replication rates, reduced cutaneous blood flow, reducedmoisture content, accumulated errors in structure and function,alterations in the normal regulation of common biochemical pathways, anda reduction in the skin's ability to remodel and repair itself. Many ofthe alterations in appearance and function of the skin are caused bychanges in the outer epidermal layer of the skin, while others arecaused by changes in the lower dermis.

One problem associated with existing skin care products is that theytend to focus on a particular skin condition without consideration ofother skin conditions. This results in a user having to purchase severaldifferent products and apply several different products throughout theday to obtain the desired results. Further, the majority of suchproducts are designed to be used throughout the daytime, which is aperiod of time that the skin is actively beings assaulted with causticenvironmental factors such as UV light, pollution, chemicals, smoke,etc. Therefore, the skin may not be completely receptive to the benefitsthat a skin composition can provide.

SUMMARY OF THE INVENTION

While the compositions of the present invention have the ability totreat skin during the daytime, the inventors discovered that a uniquecombination of skin active ingredients (e.g., palmitoyl tetrapeptide-7,methylsilanol mannuronate, Lactobacillus ferment, and plant extractsfrom Punica granatum, Castanea sativa, Gossypium hirsutum, and Euterpeoleracea) works surprisingly well in treating skin during the eveninghours. This combination of ingredients is designed to work in sync witha person's skin rhythms to help the skin recover/recuperate during sleepmode.

In one aspect, there is disclosed a method of treating skin duringsleep. The method can include topically applying a composition to auser's skin prior to the user falling asleep. The composition caninclude a chemically compatible combination of skin active ingredientscomprising palmitoyl tetrapeptide-7, methylsilanol mannuronate, andLactobacillus ferment, and a chemically compatible combination of skinactive ingredients comprising plant extracts from Punica granatum,Castanea sativa, Gossypium hirsutum, and Euterpe oleracea, and adermatologically acceptable vehicle, a vehicle which can have hydratingand/or moisturization properties. The method can further includeremoving the composition from the user's skin after the user wakes up.This can result in rejuvenation or replenishing the skin during theevening hours by using the unique combination of ingredients. Thiscombination is proven to be chemically compatible (i.e., they are ableto coexist together without detrimentally affecting their individualskin efficacy abilities) and skin friendly in that the combination doesnot appear to irritate the skin. The result of this combination is asuperior way to rejuvenate the skin during the evening hours, whichultimately provides for an effective way to treat a wide variety of skinconditions such as firming/toning the skin, increasing the skin'selasticity, reducing the appearance of dark spots or aged spots, eveningout the skin's tone, reducing the appearance of fine lines and wrinkles,reducing other signs of premature skin aging, and reducing theappearance of expression lines. The evening hours typically includes thetime the sun goes down to the time the sun comes up. The composition canbe applied during the evening hours prior to falling asleep and can beremoved when the user awakens. The composition can be applied to alltypes of skin such as the face, neck, decolette, arms, hands, body,legs, feet, etc. The composition can be formulated as a cream, lotion,gel, gel-like cream, serum, etc. The color of the composition can beopaque, transparent, translucent, etc. The composition can be formulatedfor use on dry skin, normal skin, oily skin, combination skin, etc. Thedermatologically acceptable vehicle can be formulated to moisturizeskin, hydrate skin, to provide a substantive effect in that thecomposition has the ability to remain on the skin even during sleepmode. In one aspect, the dermatologically acceptable vehicle comprisesat least 50% by weight of water, 3 to 10% by weight of glycerin, 3 to10% by weight of butylene glycol, 1 to 3% by weight of glycerylstearate, 1 to 5% by weight of caprylic/capric triglyceride, and 1 to 5%by weight of hydrogenated polydecene. In another aspect, thedermatologically acceptable vehicle comprises water, glycerin, butyleneglycol, glyceryl stearate, caprylic/capric triglyceride, andhydrogenated polydecene. The ratio of water to glycerin can be importantfor skin types. In some instances, the water:glycerine ratio is about5:1 to about 20:1 (or 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1,15:1, 16:1, 17:1, 18:1, 19:1 or any range therein) based on the totalweight of the composition. The composition may not have a sunscreenagent, a cyclomethicone, an alpha hydroxy acid, a beta hydroxy acid, anoil, an acid or base based molecule, a vitamin, and/or any other skinactive ingredients or other skin active ingredients in skin activeamounts.

Also disclosed in a topical skin care composition comprising achemically compatible combination of skin active ingredients comprisingpalmitoyl tetrapeptide-7, methylsilanol mannuronate, and Lactobacillusferment, a chemically compatible combination of skin active ingredientscomprising plant extracts from Punica granatum, Castanea sativa,Gossypium hirsutum, and Euterpe oleracea, and a dermatologicallyacceptable vehicle. The dermatologically acceptable vehicle can includeat least 50% by weight of water, 3 to 10% by weight of glycerin, 3 to10% by weight of butylene glycol, 1 to 3% by weight of glycerylstearate, 1 to 5% by weight of caprylic/capric triglyceride, and 1 to 5%by weight of hydrogenated polydecene. The dermatologically acceptablevehicle can include water, glycerin, butylene glycol, glyceryl stearate,caprylic/capric triglyceride, and hydrogenated polydecene. In certaininstances, the water: glycerine ratio is about 5:1 to about 20:1 (or6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1,18:1, 19:1 or any range therein) based on the total weight of thecomposition. In further aspects, the dermatologically acceptable vehiclecan include water, glycerin, a combination of butylene glycol andpropylene glycol, glyceryl stearate, dimethicone, disodium EDTA,steareth-20, polyethylene glycol-40 stearate, caprylic/caprictriglyceride, hydrogenated polydecene, methyl trimethicone,butyrospermum parkii, sodium polyacrylate, and butylated hydroxytolene.The composition can be an emulsion, a gel, a cream, a lotion, a serum,etc. The composition can be translucent, transparent, or opaque. Thecomposition can be formulated for normal, dry, oily, or combinationskin-types. The composition may not have a sunscreen agent, acyclomethicone, an alpha hydroxy acid, a beta hydroxy acid, an oil, anacid or base based molecule, a vitamin, and/or any other skin activeingredients or other skin active ingredients in skin active amounts.

In still another aspect, there is disclosed a combination of palmitoyltetrapeptide-7, methylsilanol mannuronate, Lactobacillus ferment, andplant extracts from Punica granatum, Castanea sativa, Gossypiumhirsutum, and Euterpe oleracea. The combination can be in an aqueoussolution, a hydrophobic solution, an alcohol solution, a glycolicsolution, in powdered form, etc. The combination can be placed incompositions for skin, food, medicines, pharmaceuticals, injectibles,etc.

In one embodiment, there is disclosed a method of firming skin orreducing the appearance of fine lines or wrinkles comprising topicallyapplying any one of the compositions of the present invention to skin inneed of such treatment. The composition can include a chemicallycompatible combination of skin active ingredients comprising palmitoyltetrapeptide-7, methylsilanol mannuronate, and Lactobacillus ferment, achemically compatible combination of skin active ingredients comprisingplant extracts from Punica granatum, Castanea sativa, Gossypiumhirsutum, and Euterpe oleracea, and a dermatologically acceptablevehicle. The composition can be applied to fine lines or wrinkles,sagging skin, skin areas prone to sagging, etc.

In an alternative embodiment of the present invention there is disclosedcompositions of the present invention can include Hydrolyzed myrtuscommunis (myrtle) leaf extract, Cucurbita pepo (pumpkin) seed extract,Gossypium hirsutum (cotton) extract, Castanea sativa (chestnut) seedextract, Euterpe oleracea (acai) fruit extract, Punica granatum(pomegranate) sterols or fruit extract, Terminalia ferdinandiana (kakaduplum) fruit extract, Ferula foetida (ferula) root extract, MyriciariaDubia (camu camu) fruit extract, Palmitoyl tetrapeptide-3,Monomethylsilanetriol mannuronate, Lactobacillus ferment extract, orAlteromonas ferment extract, or combinations thereof. In one instance,the combination includes palmitoyl tetrapeptide-7, methylsilanolmannuronate, Lactobacillus ferment, and plant extracts from Punicagranatum, Castanea sativa, Gossypium hirsutum, and Euterpe oleracea.Additional ingredients such as Helianthus annus (sunflower) seedextract, dipotassium glycyrrhizate, ascorbyl glucoside, Argania spinosa(argan) seed kernel extract, Secale cereale (rye) seed extract, Linumusitatissimum (linseed) seed extract, Malpighia punicifolia (acerola)fruit extract, Pinus sylvestris bark extract, Ribes nigrum (blackcurrant) leaf extract, palmitoyl oligopeptide, palmitoyl tetrapeptide-7,or adenosine, or any combination thereof, can also be incorporated intothe compositions of the present invention. Further, as shown in thefigures and examples (which are incorporated into this section byreference), the inventors have discovered that the combination of anyone of Hydrolyzed myrtus communis (myrtle) leaf extract, Cucurbita pepo(pumpkin) seed extract, Gossypium hirsutum (cotton) extract, Castaneasativa (chestnut) seed extract, Euterpe oleracea (acai) fruit extract,Punica granatum (pomegranate) sterols or fruit extract, Terminaliaferdinandiana (kakadu plum) fruit extract, Ferula foetida (ferula) rootextract, Myriciaria Dubia (camu camu) fruit extract, Palmitoyltetrapeptide-3, Monomethylsilanetriol mannuronate, Lactobacillus fermentextract, or Alteromonas ferment extract, or combinations thereof,produce synergistic and complimentary effects that are beneficial toskin.

In certain embodiments, the compositions are formulated into topicalskin care compositions. The compositions can be cosmetic compositions.In other aspects, the compositions can be included in a cosmeticvehicle. Non-limiting examples of cosmetic vehicles are disclosed inother sections of this specification and are known to those of skill inthe art. Examples of cosmetic vehicles include emulsions (e.g.,oil-in-water and water-in-oil emulsions), creams, lotions, solutions(e.g., aqueous or hydro-alcoholic solutions), anhydrous bases (e.g.,lipstick or a powder), gels, and ointments. In other non-limitingembodiments, the compositions of the present invention can be includedin anti-aging, cleansing, or moisturizing products. The compositions canalso be formulated for topical skin application at least 1, 2, 3, 4, 5,6, 7, or more times a day during use. In other aspects of the presentinvention, compositions can be storage stable or color stable, or both.It is also contemplated that the viscosity of the composition can beselected to achieve a desired result (e.g., depending on the type ofcomposition desired, the viscosity of such composition can be from about1 cps to well over 1 million cps or any range or integer derivabletherein (e.g., 2 cps, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70,80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 2000, 3000,4000, 5000, 6000, 7000, 8000, 9000, 10000, 20000, 30000, 40000, 50000,60000, 70000, 80000, 90000, 100000, 200000, 300000, 400000, 500000,600000, 700000, 800000, 900000, 1000000 cps, etc., as measured on aBrookfield Viscometer using a TC spindle at 2.5 rpm at 25° C.).

The compositions in non-limiting aspects can have a pH of about 6 toabout 9. In other aspects, the pH can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10,11, 12, 13, or 14. Compositions of the present invention can have UVAand UVB absorption properties. The compositions can have an sunprotection factor (SPF) of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 20, 25, 30, 35, 40, 45, 50, 55, 60, or more, or any integer orderivative therein. The compositions can be sunscreen lotions, sprays,or creams.

The compositions of the present invention can also be modified to have adesired oxygen radical absorbance capacity (ORAC) value. In certainnon-limiting aspects, the compositions of the present invention can bemodified to have an ORAC value per mg of at least about 1, 2, 3, 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24,25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 70, 80, 90, 95, 100,200, 300, 400, 500, 600, 700, 800, 900, 1000, 2000, 3000, 4000, 5000,6000, 7000, 8000, 9000, 10000, 15000, 20000, 30000, 50000, 100000 ormore or any range derivable therein.

In particular aspects, the compositions can be oil-free, substantiallyanhydrous, and/or anhydrous. Other aspects include compositions havingwater.

In one aspect of the present invention, there is disclosed a topicalskin care composition that include a skin active ingredient selectedfrom the group consisting of: Hydrolyzed myrtus communis (myrtle) leafextract; Cucurbita pepo (pumpkin) seed extract; Gossypium hirsutum(cotton) extract; Castanea sativa (chestnut) seed extract; Euterpeoleracea (acai) fruit extract; Punica granatum (pomegranate) sterols orfruit extract; Terminalia ferdinandiana (kakadu plum) fruit extract;Ferula foetida (ferula) root extract; Myriciaria Dubia (camu camu) fruitextract; Palmitoyl tetrapeptide-3; Monomethylsilanetriol mannuronate;Lactobacillus ferment extract; and Alteromonas ferment extract; and anycombination of such skin active ingredients. In one instance, thecombination includes palmitoyl tetrapeptide-7, methylsilanolmannuronate, Lactobacillus ferment, and plant extracts from Punicagranatum, Castanea sativa, Gossypium hirsutum, and Euterpe oleracea. Incertain aspects, the compositions can include all of these skin activeingredients. The compositions of the present invention can also includeany one of, any combination of, or all of the following additionalingredients: water, a chelating agent, a moisturizing agent, apreservative, a thickening agent, a silicone containing compound, anessential oil, a structuring agent, a vitamin, a pharmaceuticalingredient, or an antioxidant, or any combination of such ingredients ormixtures of such ingredients. In certain aspects, the composition caninclude at least two, three, four, five, six, seven, eight, nine, ten,or all of these additional ingredients identified in the previoussentence. Non-limiting examples of these additional ingredients areidentified throughout this specification and are incorporated into thissection by reference. The amounts of such ingredients can range from0.0001% to 99.9% by weight or volume of the composition, or any integeror range in between as disclosed in other sections of thisspecification, which are incorporated into this paragraph by reference.

In another embodiment, there is disclosed a topical skin carecomposition comprising: palmitoyl tetrapeptide 7; Euterpe oleracea(acai) fruit extract or Terminalia ferdinandiana (kakadu plum) fruitextract or a combination thereof; and a dermatologically acceptablevehicle comprising any one of or all of the following: water; glycerin;butylene glycol; propylene glycol; and disodium EDTA, wherein thetopical skin care composition is formulated for topical application toskin. In particular aspects, the composition includes any one of or allof the following: 30 to 80% by weight of water; 3 to 25% by weight ofglycerin; 0.01 to 5% by weight of butylene glycol; 0.1 to 1% by weightof propylene glycol; and 0.01 to 0.5% by weight of disodium EDTA. Inother aspects, the ratio of water to glycerin can be from about 0.5:1 toabout 20:1 or anything in between (e.g., 1:1, 2:1, 3:1, 4:1, 5:1, 6:1,7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, or19:1, or any range therein) based on the total weight of thecomposition. In certain aspects, the range can be from about 6:1 toabout 20:1, whereas in other aspects it can be about 0.5:1 to about 2:1.In other aspects, the ratio of propylene glycol to disodium EDTA in thecomposition can be from about 1:1 to about 30:1 or anything in between(e.g., 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1,13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1,25:1, 26:1, 27:1, 28:1, 29:1 or any range therein) based on the totalweight of the composition. In particular embodiments, the ratio ofpropylene glycol to disodium EDTA can be from about 1.5:1 to about 3.5:1based on the total weight of the composition or from about 15:1 to about30:1 based on the total weight of the composition. The composition canfurther include any one of or all of the following: phenoxyethanol;steareth-20; chlorhexidine diglunonate; potassium sorbate; and apreservative selected from the group consisting of methyparaben,propylparaben, butylparaben, ethylparaben, and isobutylparaben, and anycombination thereof. In certain aspects, the composition can include anyone of or all of the following: 0.00001 to 1% by weight ofphenoxyethanol; 0.0001 to 0.1% by weight of steareth-20; 0.00001 to 0.1%by weight of chlorhexidine diglunonate; 0.000001 to 0.01% by weight ofpotassium sorbate; and 0.000001 to 1% by weight of a preservativeselected from the group consisting of methyparaben, propylparaben,butylparaben, ethylparaben, and isobutylparaben, and any combinationthereof. In certain aspects, the composition can further include any oneof or all of the following skin active ingredients:

Hydrolyzed myrtus communis (myrtle) leaf extract; Cucurbita pepo(pumpkin) seed extract; Gossypium hirsutum (cotton) extract; Castaneasativa (chestnut) seed extract; Punica granatum (pomegranate) sterols orfruit extract; Ferula foetida (ferula) root extract; Myriciaria Dubia(camu camu) fruit extract; methylsilanol mannuronate; Lactobacillusferment extract; or Alteromonas ferment extract; or any combinationthereof. The amount of any one of the aforementioned ingredients withina given composition can vary to as little as 0.0000001% to 99.9% or anyrange or integer divisible therein as discussed in other sections of thespecification, which are incorporated by reference.

In particular aspects of the present invention, there is disclosed atopical skin care composition that includes any one of, any combinationof, or all of the following ingredients: palmitoyl tetrapeptide 7;Euterpe oleracea (acai) fruit extract or Terminalia ferdinandiana(kakadu plum) fruit extract or a combination thereof; water; glycerin;butylene glycol; propylene glycol; disodium EDTA; phenoxyethanol;steareth-20; chlorhexidine diglunonate; potassium sorbate; apreservative; Cucurbita pepo (pumpkin) seed extract; Lactobaccillusferment extract; Myriciaria Dubia (camu camu) fruit extract; Hydrolyzedmyrtus communis (myrtle) leaf extract; Alteromonas ferment extract; orFerula foetida (ferula) root extract. The composition can include 40 to50% by weight of water, 3 to 5% by weight of glycerin, and 2 to 5% byweight of butylene glycol. The composition can further include any oneof, any combination of, or all of the following: denatured alcohol;behenyl alcohol; nylon-12; ethylene/acrylic acid copolymer; glycerylstearate; or aluminum starch octenylsuccinate. The amounts of suchingredients can be 2 to 5% by weight of denatured alcohol; 1 to 3% byweight of behenyl alcohol; 1 to 3% by weight of nylon-12; 1 to 3% byweight of ethylene/acrylic acid copolymer; 0.1 to 2% by weight ofglyceryl stearate; or 0.1 to 2% by weight of aluminum starchoctenylsuccinate. The composition can further include a UV absorbingagent selected from the group consisting of homosalate, octisalate,oxybenzone, and avobenzone, and any combination thereof.

In still another embodiment, there is disclosed a topical skin carecomposition that includes any one of, any combination of, or all of thefollowing ingredients: palmitoyl tetrapeptide 7; Euterpe oleracea (acai)fruit extract or Terminalia ferdinandiana (kakadu plum) fruit extract ora combination thereof; water; glycerin; butylene glycol; propyleneglycol; disodium EDTA; phenoxyethanol; steareth-20; chlorhexidinediglunonate; potassium sorbate; a preservative; Cucurbita pepo (pumpkin)seed extract; Lactobaccillus ferment extract; Myriciaria Dubia (camucamu) fruit extract; Hydrolyzed myrtus communis (myrtle) leaf extract;Alteromonas ferment extract; or Ferula foetida (ferula) root extract.The composition can include 40 to 50% by weight of water, 3 to 5% byweight of glycerin, and 2 to 5% by weight of butylene glycol. Thecomposition can further include any one of, any combination of, or allof the following: denatured alcohol; behenyl alcohol; ethylene/acrylicacid copolymer; glyceryl stearate; or aluminum starch octenylsuccinate.The amount of such ingredients can be 2 to 5% by weight of denaturedalcohol; 1 to 3% by weight of behenyl alcohol; 1 to 3% by weight ofethylene/acrylic acid copolymer; 0.1 to 2% by weight of glycerylstearate; and 0.1 to 2% by weight of aluminum starch octenylsuccinate.The composition can further include a UV absorbing agent selected fromthe group consisting of homosalate, octisalate, oxybenzone, andavobenzone, and any combination thereof. The composition can beformulated as an oil-in-water emulsion.

In even another aspect, there is disclosed a topical skin carecomposition that includes any one of, any combination of, or all of thefollowing ingredients: palmitoyl tetrapeptide 7; Euterpe oleracea (acai)fruit extract or Terminalia ferdinandiana (kakadu plum) fruit extract ora combination thereof; water; glycerin; butylene glycol; propyleneglycol; disodium EDTA; phenoxyethanol; steareth-20; chlorhexidinediglunonate; potassium sorbate; a preservative; Myriciaria Dubia (camucamu) fruit extract; or Hydrolyzed myrtus communis (myrtle) leafextract. The topical skin care composition can include 35 to 45% byweight of water and 20 to 30% by weight of glycerin. The composition canfurther include any one of, any combination of, or all of the followingadditional ingredients: potassium myristate; cocamidopropyl betaine;myristic acid; potassium laurate; sodium myristoyl glutamate; PEG-32;sorbitol; or glyceryl stearate. The composition can include 5 to 10% byweight of potassium myristate; 3 to 7% by weight of cocamidopropylbetaine; 2 to 5% by weight of myristic acid; 2 to 5% by weight ofpotassium laurate; 2 to 5% by weight of sodium myristoyl glutamate; 1 to3% by weight of PEG-32; 1 to 3% by weight of sorbitol; and 1 to 3% byweight of glyceryl stearate.

In still another embodiment, there is disclosed a topical skin carecomposition that includes any one of, any combination of, or all of thefollowing ingredients: palmitoyl tetrapeptide 7; Euterpe oleracea (acai)fruit extract or Terminalia ferdinandiana (kakadu plum) fruit extract ora combination thereof; water; glycerin; butylene glycol; propyleneglycol; disodium EDTA; phenoxyethanol; steareth-20; chlorhexidinediglunonate; potassium sorbate; a preservative; Myriciaria Dubia (camucamu) fruit extract; or Hydrolyzed myrtus communis (myrtle) leafextract. The topical skin care composition can include 30 to 40% byweight of water and 35 to 45% by weight of glycerin. The topical skincare composition can further include any one of, any combination of, orall of the following: potassium myristate; potassium laurate; myristicacid; stearic acid; sodium chloride; potassium stearate; PEG-40stearate; sodium methyl cocoyl taurate; lauric acid; glycol stearate; orsodium cocyl glycinate. The composition can include 3 to 7% by weight ofpotassium myristate; 2 to 5% by weight of potassium laurate; 0.5 to 2%by weight of myristic acid; 1 to 3% by weight of stearic acid; 0.5 to 2%by weight of sodium chloride; 2 to 5% by weight of potassium stearate; 1to 5% by weight of PEG-40 stearate; 1 to 3% by weight of sodium methylcocoyl taurate; 0.5 to 2% by weight of lauric acid; 0.5 to 2% by weightof glycol stearate; and 0.5 to 2% by weight of sodium cocyl glycinate.

In yet another embodiment, there is disclosed a topical skin carecomposition that includes any one of, any combination of, or all of thefollowing ingredients: palmitoyl tetrapeptide 7; Euterpe oleracea (acai)fruit extract or Terminalia ferdinandiana (kakadu plum) fruit extract ora combination thereof; water; glycerin; butylene glycol; propyleneglycol; disodium EDTA; phenoxyethanol; steareth-20; chlorhexidinediglunonate; potassium sorbate; a preservative; Punica granatum(pomegranate) sterols or fruit extract; Castanea sativa (chestnut) seedextract; Gossypium hirsutum (cotton) extract; or methylsilanolmannuronate. The composition can include 65 to 75% by weight of water, 3to 5% by weight of glycerin, and 7 to 12% by weight of butylene glycol.The composition can further include any one of, any combination of, orall of the following: glyceryl stearate; caprylic/capric triglyceride;or hydrogenated polydecene. The composition can include 0.5 to 2% byweight glyceryl stearate; 3 to 7% by weight of caprylic/caprictriglyceride; and 1 to 3% by weight of hydrogenated polydecene. Thetopical skin care composition can be formulated as a water-in-oilemulsion.

In another embodiment, there is disclosed a topical skin carecomposition that includes any one of, any combination of, or all of thefollowing ingredients: palmitoyl tetrapeptide 7; Euterpe oleracea (acai)fruit extract or Terminalia ferdinandiana (kakadu plum) fruit extract ora combination thereof; water; glycerin; butylene glycol; propyleneglycol; disodium EDTA; phenoxyethanol; steareth-20; chlorhexidinediglunonate; potassium sorbate; a preservative; Lactobacillus fermentextract; Punica granatum (pomegranate) sterols or fruit extract;Castanea sativa (chestnut) seed extract; Gossypium hirsutum (cotton)extract; or methylsilanol mannuronate. The composition can include 55 to65% by weight of water, 5 to 10% by weight of glycerin, and 2 to 5% byweight of butylene glycol. The composition can further include any oneof, any combination of, or all of the following: glyceryl stearate;dimethicone; PEG-40 stearate; caprylic/capric triglyceride; hydrogenatedpolydecene; methyl trimethicone; shea butter; a biosaccharide gum; oroctyldodecyl myristate. The composition can include 0.5 to 2% by weightof glyceryl stearate; 2 to 5% by weight of dimethicone; 0.5 to 2% byweight of PEG-40 stearate; 2 to 5% by weight of caprylic/caprictriglyceride; 3 to 7% by weight of hydrogenated polydecene; 2 to 5% byweight of methyl trimethicone; 2 to 5% by weight of shea butter; 1 to 5%by weight of a biosaccharide gum; and 1 to 3% by weight of octyldodecylmyristate. The composition can be formulated as a water-in-oil emulsion.

In a particular embodiment, there is disclosed an oil-in-water emulsioncomprising any one of, any combination of, or all of the followingingredients: water; glycerin; butylene glycol; sodium polyacrylate;disodium EDTA; caprylic/capric triglyceride; cyclomethicone; glycerylstearate; dimethicone; cetearyl alcohol; C12-22 alcohols; c12-20 alkylglucoside; a preservative, and skin active ingredient. The amount ofsuch ingredients can vary as disclosed throughout this specification.Non-limiting ranges of the amounts of such ingredients in a formulation(either by weight or volume) include: water (q.s. to 100%); glycerin (3%to 5%); butylene glycol (5% to 10%); sodium polyacrylate (0.1% to 1%);disodium EDTA (0.01% to 1%); caprylic/capric triglyceride (1% to 7%);cyclomethicone (0.01% to 3%); glyceryl stearate (0.5% to 3%);dimethicone (0.1% to 2%); cetearyl alcohol (0.1% to 2%); C12-22 alcohols(0.1% to 3%); C12-20 alkyl glucoside (0.01% to 1%); a preservative(0.01% to 5%), and skin active ingredient (0.001% to 5%). Additionalingredients can also be included, non-limiting examples of whichinclude: 2 to 5% by weight of denatured alcohol; 1 to 3% by weight ofbehenyl alcohol; 1 to 3% by weight of nylon-12; 1 to 3% by weight ofethylene/acrylic acid copolymer; 0.1 to 2% by weight of glycerylstearate; 0.1 to 2% by weight of aluminum starch octenylsuccinate;and/or a combination of Cucurbita pepo (pumpkin) seed extract,Lactobaccillus ferment extract, Terminalia ferdinandiana (kakadu plum)fruit extract, Myriciaria Dubia (camu camu) fruit extract, Hydrolyzedmyrtus communis (myrtle) leaf extract, Alteromonas ferment extract,and/or Ferula foetida (ferula) root extract; or any combination thereof.

In another particular embodiment, there is disclosed an oil-in-wateremulsion comprising any one of, any combination of, or all of thefollowing ingredients: water; glycerin; butylene glycol; disodium EDTA;acrylates copolymer; polyacrylamide; glyceryl stearate and PEG-100stearate; cetyl alcohol; C13-14 isoparaffin; laureth-7; cetearylalcohol; ceteth-20 phosphate; dicetyl phosphate; homosalate;dimethicone; octisalate; oxybenzone; avobenzone; preservative; skinactives; and additional ingredients. Non-limiting ranges of the amountsof such ingredients in a formulation (either by weight or volume)include: water (q.s. to 100%); glycerin (3% to 5%); butylene glycol (1%to 3%); disodium EDTA (0.01% to 1%); acrylates copolymer (1% to 3%);polyacrylamide (0.01% to 1%); glyceryl stearate and PEG-100 stearate (1%to 3%); cetyl alcohol (1% to 3%); C13-14 isoparaffin (0.01% to 1%);laureth-7 (0.01% to 1%); cetearyl alcohol (0.01% to 1%); ceteth-20phosphate (0.1% to 1%); dicetyl phosphate (0.01% to 1%); homosalate (5%to 15%); dimethicone (0.01% to 1%); octisalate (3% to 8%); oxybenzone(3% to 8%); avobenzone (1% to 5%); preservative (0.01% to 5%); and skinactives (0.01% to 5%). Additional ingredients can also be included,non-limiting examples of which include: 2 to 5% by weight of denaturedalcohol; 1 to 3% by weight of behenyl alcohol; 1 to 3% by weight ofethylene/acrylic acid copolymer; 0.1 to 2% by weight of glycerylstearate; 0.1 to 2% by weight of aluminum starch octenylsuccinate;and/or a combination of Cucurbita pepo (pumpkin) seed extract,Lactobaccillus ferment extract, Terminalia ferdinandiana (kakadu plum)fruit extract, Myriciaria Dubia (camu camu) fruit extract, Hydrolyzedmyrtus communis (myrtle) leaf extract, Alteromonas ferment extract,and/or Ferula foetida (ferula) root extract; or any combination thereof.

In still another particular embodiment, there is disclosed a cleansercomprising any one of, any combination of, or all of the followingingredients: water; glycerin; cocamidopropyl betaine; potassiummyristate; stearic acid; disodium EDTA; triethanolamine; preservatives;skin active ingredients; and additional ingredients. Non-limiting rangesof the amounts of such ingredients in a formulation (either by weight orvolume) include: water (q.s. to 100%); glycerin (20% to 30%);cocamidopropyl betaine (10% to 20%); potassium myristate (5% to 10%);stearic acid (1% to 3%); disodium EDTA (0.01% to 1%); triethanolamine(0.01% to 1%); preservative (0.01% to 5%); and skin actives (0.01% to5%). Additional ingredients can also be included, non-limiting examplesof which include: 2 to 5% by weight of potassium laurate; 2 to 5% byweight of myristic acid; 2 to 5% by weight of sodium myristoylglutamate; 1 to 3% by weight of PEG-32; 1 to 3% by weight of sorbitol; 1to 3% by weight of glyceryl stearate; and/or a combination of Terminaliaferdinandiana (kakadu plum) fruit extract, Myriciaria Dubia (camu camu)fruit extract, and/or Hydrolyzed myrtus communis (myrtle) leaf extract;or any combination thereof.

Also disclosed is a method of treating or preventing a skin conditioncomprising topically applying any composition disclosed throughout thespecification and claims to skin having a skin condition or at risk ofhaving a skin condition, wherein topical application of the compositionto the skin condition treats the skin condition or prevents the skincondition from forming. In particular embodiments, the skin condition isa fine line or wrinkle, dry or flaky skin, erythema, sensitive skin, orinflamed skin. In particular aspects, erythema, sensitive skin, orinflamed skin is caused by skin sunburn, electrical treatments of skin,skin burns, contact allergies, systemic allergies, skin toxicity,exercise, insect stings, bacterial infection, viral infection, fungalinfection, protozoa infection, massage, or windburn. In other aspects,the following additional skin conditions can be treated or prevented inaccordance with the methods and compositions disclosed throughout thespecification and claims: pruritus, spider veins, lentigo, age spots,senile purpura, keratosis, melasma, blotches, nodules, sun damaged skin,dermatitis (including, but not limited to seborrheic dermatitis,nummular dermatitis, contact dermatitis, atopic dermatitis, exfoliativedermatitis, perioral dermatitis, and stasis dermatitis), psoriasis,folliculitis, rosacea, acne, impetigo, erysipelas, erythrasma, eczema,and other inflammatory skin conditions. In certain non-limiting aspects,the skin condition can be caused by exposure to UV light, age,irradiation, chronic sun exposure, environmental pollutants, airpollution, wind, cold, heat, chemicals, disease pathologies, smoking, orlack of nutrition. The skin can be facial skin or non-facial skin (e.g.,arms, legs, hands, chest, back, feet, etc.). The method can furthercomprise identifying a person in need of skin treatment. The person canbe a male or female. The age of the person can be at least 1, 2, 3, 4,5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75,80, 85, 90, 95, or more years old, or any range derivable therein. Themethod can also include topically applying an amount effective to:increase the stratum corneum turnover rate of the skin; increasecollagen synthesis in fibroblasts; increase cellular anti-oxidantdefense mechanisms (e.g., exogenous additions of anti-oxidants canbolster, replenish, or prevent the loss of cellular antioxidants such ascatalase and glutathione in skin cells (e.g., keratinocytes,melanocytes, langerhans cells, etc.) which will reduce or preventoxidative damage to the skin, cellular, proteins, and lipids); inhibitmelanin production in melanocytes; reduce or prevent oxidative damage toskin (including reducing the amount lipid peroxides and/or proteinoxidation in the skin). In one instance, the composition includespalmitoyl tetrapeptide-7, methylsilanol mannuronate, Lactobacillusferment, and plant extracts from Punica granatum, Castanea sativa,Gossypium hirsutum, and Euterpe oleracea, which can treat a wide rangeof skin conditions.

Also disclosed is a method of reducing the appearance of uneven skintone comprising topically applying any one of the compositions disclosedthroughout the specification and claims to skin having an uneven skintone, wherein topical application of the composition to uneven skin tonereduces the appearance of uneven skin tone. In one instance, thecomposition includes palmitoyl tetrapeptide-7, methylsilanolmannuronate, Lactobacillus ferment, and plant extracts from Punicagranatum, Castanea sativa, Gossypium hirsutum, and Euterpe oleracea.

In another embodiment, there is disclosed a method of reducing painassociated with erythema, sensitive skin, or inflamed skin, comprisingtopically applying any one of the compositions disclosed throughout thespecification and claims to erythemic, sensitive, or inflamed skin,wherein topical application of the composition to erythemic, sensitive,or inflamed skin reduces the pain associated with erythema, sensitiveskin, or inflamed skin. In one instance, the composition includespalmitoyl tetrapeptide-7, methylsilanol mannuronate, Lactobacillusferment, and plant extracts from Punica granatum, Castanea sativa,Gossypium hirsutum, and Euterpe oleracea.

In still another aspect, there is disclosed a method of reducing theappearance of symptoms associated with erythema, sensitive skin, orinflamed skin, comprising topically applying any one of the compositionsdisclosed throughout the specification and claims erythemic, sensitive,or inflamed skin, wherein topical application of the composition toerythemic, sensitive, or inflamed skin reduces the appearance ofsymptoms associated with erythema, sensitive skin, or inflamed skin. Inone instance, the composition includes palmitoyl tetrapeptide-7,methylsilanol mannuronate, Lactobacillus ferment, and plant extractsfrom Punica granatum, Castanea sativa, Gossypium hirsutum, and Euterpeoleracea.

Also disclosed is a method of removing dirt, oil, or make-up from skincomprising: applying any one of the compositions disclosed throughoutthe specification and claims to skin in need of removal of dirt, oil, ormake-up; and removing the composition from the skin with water withinabout 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 30, 40, 50,60, 120, 180, or minutes after application, wherein dirt, oil, or makeupis removed from the skin. In particular aspects, the composition isapplied to dirt on the skin, and wherein the dirt is removed from theskin. In other aspects, the composition is applied to oil on the skin,and wherein the oil is removed from the skin. In another embodiment, thecomposition is applied to make-up on the skin, and wherein the make-upis removed from the skin.

In other aspect, there is disclosed a method of increasing collagenproduction in a skin cell comprising topically applying any one of thecompositions disclosed throughout the specification and claims to a skincell in need of collagen production, wherein the topical application ofthe composition to the skin cell increases collagen production in theskin cell. Non-limiting examples of such cells include human epidermalkeratinocyte, human fibroblast dermal cell, human melanocytes, threedimensional human cell-derived in vitro tissue equivalents comprisinghuman keratinocytes, human fibroblasts, or human melanocytes, or anycombination thereof (e.g., combination of human keratinocytes and humanfibroblasts or a combination of human keratinocytes and humanmelanocytes). In one instance, the composition includes palmitoyltetrapeptide-7, methylsilanol mannuronate, Lactobacillus ferment, andplant extracts from Punica granatum, Castanea sativa, Gossypiumhirsutum, and Euterpe oleracea.

Also disclosed is a method of lightening skin or evening skin tonecomprising applying any one of the compositions disclosed throughout thespecification and claims to skin. The method can further compriseidentify a person in need of lightening skin or evening skin tone. Themethods can further include inhibiting melanogenesis in a skin cell,inhibiting tyrosinase or tyrosinase synthesis in a skin cell, orinhibiting melanin transport to keratinocytes in a skin cell. Thecomposition can act as an alpha melanin stimulatory hormone antagonist.The composition can even out pigmentation of the skin. In non-limitingaspect, lightening skin can include reducing the appearance of an agespot, a skin discoloration, or a freckle by topical application of thecomposition to skin having an age spot, skin discoloration, a freckle,etc. In one instance, the composition includes palmitoyl tetrapeptide-7,methylsilanol mannuronate, Lactobacillus ferment, and plant extractsfrom Punica granatum, Castanea sativa, Gossypium hirsutum, and Euterpeoleracea.

Also disclosed is a method of treating hyperpigmentation comprisingapplying any one of the compositions disclosed throughout thespecification and claims to skin. The method can also compriseidentifying a person in need of treating hyperpigmentation. Additionalmethods contemplated by the inventor include methods for reducing theappearance of an age spot, a skin discoloration, or a freckle, reducingor preventing the appearance of fine lines or wrinkles in skin, orincreasing the firmness of skin. In one instance, the compositionincludes palmitoyl tetrapeptide-7, methylsilanol mannuronate,Lactobacillus ferment, and plant extracts from Punica granatum, Castaneasativa, Gossypium hirsutum, and Euterpe oleracea.

Also contemplated are kits that include any one of the compositionsdisclosed throughout the specification and claims. In certainembodiments, the composition is comprised in a container. The containercan be a bottle, dispenser, or package. The container can dispense apre-determined amount of the composition. In certain aspects, thecompositions is dispensed in a spray, dollop, or liquid. The containercan include indicia on its surface. The indicia can be a word, anabbreviation, a picture, or a symbol.

Also contemplated is a product comprising a composition of the presentinvention. In non-limiting aspects, the product can be a cosmeticproduct. The cosmetic product can be those described in other sectionsof this specification or those known to a person of skill in the art.Non-limiting examples of products include a moisturizer, a cream, alotion, a skin softener, a foundation, a night cream, a lipstick, acleanser, a toner, a sunscreen, a mask, or an anti-aging product.

The compositions and methods for their use can “comprise,” “consistessentially of,” or “consist of” any of the ingredients disclosedthroughout the specification. For purposes of consisting essentially ofmeans that inclusion of additional ingredients in the compositions donot materially affect the multi-beneficial properties of the combinationof palmitoyl tetrapeptide-7, methylsilanol mannuronate, Lactobacillusferment, and plant extracts from Punica granatum, Castanea sativa,Gossypium hirsutum, and Euterpe oleracea. One such instance would be theinclusion of an ingredient that has a detrimental effect (e.g., reducingthe efficacy or stability) on any one of the ingredients identified inthe combination.

It is contemplated that any embodiment discussed in this specificationcan be implemented with respect to any method or composition of theinvention, and vice versa. Furthermore, compositions of the inventioncan be used to achieve methods of the invention.

In one embodiment, compositions of the present invention can bepharmaceutically or cosmetically elegant. “Pharmaceutically elegant”and/or “cosmetically elegant” describes a composition that hasparticular tactile properties which feel pleasant on the skin (e.g.,compositions that are not too watery or greasy, compositions that have asilky texture, compositions that are non-tacky or sticky, etc.).Pharmaceutically or cosmetically elegant can also relate to thecreaminess or lubricity properties of the composition or to the moistureretaining properties of the composition.

“Topical application” means to apply or spread a composition onto thesurface of keratinous tissue. “Topical skin composition” includescompositions suitable for topical application on keratinous tissue. Suchcompositions are typically dermatologically-acceptable in that they donot have undue toxicity, incompatibility, instability, allergicresponse, and the like, when applied to skin. Topical skin carecompositions of the present invention can have a selected viscosity toavoid significant dripping or pooling after application to skin.

“Keratinous tissue” includes keratin-containing layers disposed as theoutermost protective covering of mammals and includes, but is notlimited to, skin, hair and nails.

A “non-volatile oil” includes those substance that will not evaporate atordinary or room temperature.

The terms “mixture,” “mix,” and “mixing” or any variants of these terms,when used in the claims and/or specification includes, stirring,blending, dispersing, milling, homogenizing, and other similar methods.The mixing of the components or ingredients of the disclosedcompositions can form into a solution. In other embodiments, themixtures may not form a solution. The ingredients/components can alsoexist as undissolved colloidal suspensions.

The term “about” or “approximately” are defined as being close to asunderstood by one of ordinary skill in the art, and in one non-limitingembodiment the terms are defined to be within 10%, preferably within 5%,more preferably within 1%, and most preferably within 0.5%.

The term “substantially” and its variations are defined as being largelybut not necessarily wholly what is specified as understood by one ofordinary skill in the art, and in one non-limiting embodimentsubstantially refers to ranges within 10%, within 5%, within 1%, orwithin 0.5%.

The terms “inhibiting” or “reducing” or any variation of these terms,when used in the claims and/or the specification includes any measurabledecrease or complete inhibition to achieve a desired result.

The term “effective,” as that term is used in the specification and/orclaims, means adequate to accomplish a desired, expected, or intendedresult.

The use of the word “a” or “an” when used in conjunction with the term“comprising” in the claims and/or the specification may mean “one,” butit is also consistent with the meaning of “one or more,” “at least one,”and “one or more than one.”

As used in this specification and claim(s), the words “comprising” (andany form of comprising, such as “comprise” and “comprises”), “having”(and any form of having, such as “have” and “has”), “including” (and anyform of including, such as “includes” and “include”) or “containing”(and any form of containing, such as “contains” and “contain”) areinclusive or open-ended and do not exclude additional, unrecitedelements or method steps.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and the examples,while indicating specific embodiments of the invention, are given by wayof illustration only. Additionally, it is contemplated that changes andmodifications within the spirit and scope of the invention will becomeapparent to those skilled in the art from this detailed description.

DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS

While the compositions of the present invention have the ability totreat skin during the daytime, the inventors discovered that a uniquecombination of skin active ingredients (e.g., palmitoyl tetrapeptide-7,methylsilanol mannuronate, Lactobacillus ferment, and plant extractsfrom Punica granatum, Castanea sativa, Gossypium hirsutum, and Euterpeoleracea) works surprisingly well in treating skin during the eveninghours. This combination of ingredients is designed to work in sync witha person's skin rhythms to help the skin recover/recuperate duringsleep.

Skin acts differently during the daylight hours when compared withnighttime hours. During the day, the skin is exposed to a wide varietyof environmental facts ranging from UV light, chronic sun exposure,environmental pollutants, chemicals, disease pathologies, smoking, etc.As a result of this exposure, the skin spends a majority of itsresources defending and protecting itself during the day. In the eveninghours, by comparison, the exposure to such environmental factors isreduced; this is especially true during sleep. Although the skinattempts to recover/heal from the daily stresses during the eveninghours, overtime the skin simply cannot keep up. A solution to thisproblem would be to provide a way to help the skin recover in a moreefficient manner during the evening hours.

To this end, Applicant discovered a way to help the skinrejuvinate/replenish the skin during the evening hours by using a uniquecombination of ingredients that provide key skin benefits. Thiscombination is chemically compatible (i.e., they are able to coexisttogether without detrimentally affecting their individual skin efficacyabilities) and skin friendly in that the combination does not appear toirritate the skin. This combination includes palmitoyl tetrapeptide-7,methylsilanol mannuronate, Lactobacillus ferment, and plant extractsfrom Punica granatum, Castanea sativa, Gossypium hirsutum, and Euterpeoleracea. The result of this combination is a superior way to rejuvenatethe skin during the evening hours, which ultimately provides for aneffective way to treat a wide variety of skin conditions such asfirming/toning the skin, increasing the skin's elasticity, reducing theappearance of dark spots or aged spots, evening out the skin's tone,reducing the appearance of fine lines and wrinkles, reducing other signsof premature skin aging, and reducing the appearance of expressionlines. This can be achieved through a single cosmeticformulation/product, which has the added benefit of avoiding to have touse multiple products per day at different times per day. Stated anotherway, the inventor's combination of ingredients along with adermatologically acceptable vehicle that can hydrate and moisturize theskin results in a single product that produces multiple skin benefits.

These and other aspect of the present invention are described in furtherdetail below.

A. Active Ingredients

As explained above, topical skin care compositions of the presentinvention can include Hydrolyzed myrtus communis (myrtle) leaf extract,Cucurbita pepo (pumpkin) seed extract, Gossypium hirsutum (cotton)extract, Castanea sativa (chestnut) seed extract, Euterpe oleracea(acai) fruit extract, Punica granatum (pomegranate) sterols or fruitextract, Terminalia ferdinandiana (kakadu plum) fruit extract, Ferulafoetida (ferula) root extract, Myriciaria Dubia (camu camu) fruitextract, Palmitoyl tetrapeptide-3, Monomethylsilanetriol mannuronate,Lactobacillus ferment extract, or Alteromonas ferment extract, orcombinations thereof.

In particular aspects, the combination includes palmitoyltetrapeptide-7, methylsilanol mannuronate, Lactobacillus ferment, andplant extracts from Punica granatum, Castanea sativa, Gossypiumhirsutum, and Euterpe oleracea. As shown in the Examples, thiscombination is surprisingly effective in treating a wide range of skinconditions and is particularly effective when applied to facial skinduring the evening hours. Any one of these ingredients can be obtainedfrom third party sellers.

For instance, Gossypium hirsutum (cotton) extract, extract from thecotton plant, can be purchased from Silab (France) under the trade nameHELIOMODULINE™, which was used in the Examples.

Castanea sativa (chestnut) seed extract, extract from the nut of thechestnut, can be purchased from Silab (France) under the trade nameRECOVERINE™ or from Alban Muller (France) under the trade namesBOTANICAL CHESTNUT FRUIT™, LIPIDAMI CHESTNUT FRUIT™, or PHYTAMICHESTNUT™. RECOVERINE™ was used in the Examples.

Euterpe oleracea (acai) fruit extract, extract from the fruit of acai,can be purchased from Southern Cross Botanicals Pty Ltd (NSW Australia),Amax NutraSource (USA) under the trade name ACAI FRUIT EXTRACT™, fromAssessa-Industria (Brazil) under the trade name FRULIX TF ACAI™, or fromCentroflora Group Botucatu (Brazil) under the trade name ACAI BERRYEXTRACT™. Euterpe oleracea (acai) fruit extract from Southern CrossBotanicals Pty Ltd (NSW Australia) was used in the Examples.

Punica granatum (pomegrannate) fruit extract can be purchased fromActive Organics (USA) under the trade names CO ACTIPHYTE OF POMEGRANATEAJ™, CO ACTIPHYTE OF POMEGRANATE GL™, CO ACTIPHYTE OF POMEGRANATE LIPOO™, CO ACTIPHYTE OF POMEGRANATE LIPO RS™, CO ACTIPHYTE OF POMEGRANATELIPO S™, and CO ACTIPHYTE OF POMEGRANATE LIPO SUN™. Punica granatum(pomegrannate) sterols, sterols obtained from pomegrannate fruit and/orseeds, can be purchased from Active Concepts (USA) under the trade nameABS POMEGRANATE STEROLS™. ABS POMEGRANATE STEROLS™ was used in theExamples.

Palmitoyl tetrapeptide-3 (also referred to as palmitoyl tetrapeptide-7),the reaction product of palmitic acid and tetrapepetide-7(Gly-Gln-Pro-Arg), can be purchased from Sederma (France) under thetrade names EYELISS™, HALOXYL™, MATRIXYL 3000™, and RIGIN™. RIGIN™ wasused in the Examples.

Monomethylsilanetriol mannuronate (also referred to as methylsilanolmannuronate), the ester of monomethylsilanol and oligomeric mannuronicacid. Monomethylsilanetriol mannuronate can be purchased from ExsymolS.A.M. (Monaco) under the trade name ALGISIUM C™, which was used in theExamples.

Lactobacillus ferment extract, which also includes the ferment and/orlysate of Lactobacillus, can be the ferment or lysate produced byfermentation of a given product (e.g., fruits, vegetables, trees,shrubs, plants, milk, seaweed, juice, etc.) with Lactobacillus. Examplesof Lactobacillus ferment extracts that can be used in the context of thepresent invention include those sold by Active Concepts (USA) under thetrade names AC DERMAPEPTIDE LIGHTENING™, ACB MUSTARD BIOFERMENT™, ACDERMAPEPTIDE WARMING OS™, ACB RED CLOVER BIOFERMENT™, ACB COCOABIOFERMENT™, ACB DATE PALM EXTRACT™, ACB MODIFIED YERBA SANTAGLYCOPROTEINT™, ACB YERBA SANTA GLYCOPROTEIN™, AC YERBA SANTAGLYCOPROTEIN CONCENTRATION™, AC PROBIOTIC 1™, ACB MUSHROOM EXTRACTPOWDER™, ACB MUSHROOM EXTRACT SM™, ACB GINSENG BIOFERMENT™, ACB SEA KELPBIOFERMENT™, ACB LEMON PEEL EXTRACT™, ACB YOGURT DERMA RESPITORY FACTORCT™, ACB OAT EXTRACT BETA™, ACB OLIVE LEAF EXTRACT™, ACB PAPYA ENZYMEEXTRACT™, ACB PUMPKIN ENZYME™, ACB PUMPKIN ENZYME EF™, ACB MODIFIEDPUMPKIN ENZYME™, ACB POMEGRANATE ENZYME™, ACB QUINOA EXTRACT™, ACBTOMOATO BIOFERMENT™, ACB LYCOPERSICUM BIOFERMENT™, AC COLORPLEX™, andACB WATERMELON BIOFERMENT™. In particular embodiments, Active ConceptsAC DERMAPEPTIDE LIGHTENINGT™ can be used for its skinlightening/whitening effects. The AC DERMAPEPTIDE LIGHTENING™, which wasused in the Examples, is a Lactobacillus ferment lysate filtrate whichincludes peptides that can help achieve skin lightening/whiteningaffects.

Hydrolyzed myrtus communis (myrtle) leaf extract, hydrolyzed extractfrom the leaf of myrtus communis, can be purchased from Silab (France)under the trade name LONGEVICELL™, which was used in the Examples.

Cucurbita pepo (pumpkin) seed extract, extract from pumpkin seeds, canbe purchased from Draco Natural Products Inc. (USA) under the trade namePUMPKIN EXTRACT™, Naturex (USA) under the trade name PUMPKIN SEEDGLYCOLIC EXTRACT™, or from Greentech S.A. (France) under the trade namesARP100™ and ARP 100 Huileux. ARP 100™ was used in the Examples.

Terminalia ferdinandiana (kakadu plum) fruit extract, extract obtainedfrom the fruit of the kakadu plum, can be purchased from Southern CrossBotanicals Pty Ltd (NSW, Australia), which was used in the Examples.

Ferula foetida (ferula) root extract, which includes extract from theroot Ferula foetida, can be purchase from Active Concepts (USA) underthe trade name ABS FERULA FOETIDA EXTRACT™ and from Arch Personal CareProducts (USA) under the trade names NAB ASAFETIDA BG™ and NAB ASAFETIDAEXTRACT™. ABS FERULA FOETIDA EXTRACT™ was used in the Examples.

Myrciaria dubia (camu camu) fruit extract can be purchased from AmaxNutraSource (USA) under the trade name CAMU CAMU EXTRACT™ and fromNichirei (Japan) under the trade names CAMU-CAMU EXTRACT B30™ orCAMU-CAMU EXTRACT W™.

Alteromonas ferment extract, extract from the product obtained by thefermentation of Alteromonas macleodii, can be purchased from AtriumInnovations (Canada) under the trade names ABYSSINE™, ABYSSINE 657™,ABYSSINE GL 70™, and LANACITYN™. ABYSSINE™ was used in the Examples.

Additional information and suppliers of the above-listed ingredients(and the corresponding trade names) can be found in InternationalCosmetic Ingredient Dictionary Handbook, 12^(th) Edition (2008), whichis incorporated by reference. Further, the extracts identified above canbe produced by obtaining the corresponding fruit, seed, or leaf, toproduce the extract by extraction methods which are known to those ofordinary skill in the art. The inventors also contemplate that otherportions of the substrate (e.g., Myrtus communis (myrtle), Cucurbitapepo (pumpkin), Gossypium hirsutum (cotton), Castanea sativa (chestnut),Euterpe oleracea (acai), Punica granatum (pomegranate), Terminaliaferdinandiana (kakadu plum), Ferula foetida (ferula), and MyriciariaDubia (camu camu)) producing the extract can be used in the compositionsand methods of the present invention. Non-limiting examples of the otherportions include the whole fruit, whole vegetable, whole plant, wholetree, whole bush, seed, peel, fruit, stem, bark, leaf, root, flower,petal, bulb, sap, etc. These other portions are described in theInternational Cosmetic Ingredient Dictionary Handbook, 12^(th) Edition(2008), which is incorporated by reference.

Further, a person of ordinary skill in the art would be able to isolateany one of the extracts identified above from parts of the correspondingplant by using any suitable method known in the art. In one non-limitingexample, the plant (or any part of the plant such as the leaves, stems,bark, roots, fruit, flowers or flower buds, seeds, seed pods, sap, wholeplant, etc.) can be disrupted by mechanical means which results in apuree. The puree is then processed to be substantially free ofimpurities or undesired solids. The puree can then be poured into ashallow vessel and quickly exposed to low temperature, i.e., flashfrozen, for example at −20° C. or lower, preferably under a vacuum forremoval of water content (lyophilization). The resultant extract canthen be used in the compositions of the present invention. In otheraspects, aqueous, alcoholic, or oil based extraction techniques, orcombinations thereof, can be used on the whole plant or any part thereofof (e.g., leaves, stems, bark, roots, fruit, flowers or flower buds,seeds, seed pods, sap, whole plant, etc.) to produce an extract. In sucha process, the desired part of the plant or the whole plant is crushedup (e.g., blender) and then subjected to a desired solvent (e.g., water,alcohol, water/alcohol, or oil based solvents) to obtain the desiredextract. The extract can then be stored in liquid form, lyophilized, orsubject to further processing techniques (e.g., heating, cooling, etc.).Extraction processes are well-known to those having ordinary skill inthe extract field (e.g., maceration, infusion, percolation, digestion,decoction, hot continuous extraction, aqueous-alcoholic extract, countercurrent extract, microwave assisted extraction, ultrasound extraction,supercritical fluid extracts, phytonic extract (e.g., withhydro-fluoro-carbon solvents), etc.

B. Determining Skin-Type

The primary skin types of humans are normal skin, dry skin, oily skin,and combination skin. Normal skin typically has an even tone, soft, asmooth texture, with no visible pores or blemishes, and no greasypatches or flaky areas.

Dry skin usually has a low level of sebum and can be prone toirritation. The appearance of dry skin is usually a parched look causedby the skin's inability to retain moisture. Oftentimes it feels “tight”and uncomfortable after washing and is prone to chapping, flaking, andcracking Dry skin can be exacerbated by wind, extremes of temperatureand air-conditioning, all of which cause the skin to flake, chap andfeel tight. Dry skin typically has a dull appearance.

Oily skin is typically shiny, thick and dull colored. It typically feelsoily oily and has coarse pores and pimples and other embarrassingblemishes. Oily skin usually has oil producing sebaceous glands that areoveractive and produce more oil than is needed. The oil oozes and givesthe skin a greasy shine. The pores are enlarged and the skin has acoarse look.

Combination skin is a combination of both oily and dry skin. Usually,there is a greasy center panel consisting of nose, forehead and chin anda dry panel consisting of cheeks, mouth and the areas around the eyes.

C. Oxygen Radical Absorbance Capacity

Oxygen Radical Absorption (or Absorbance) Capacity (ORAC) is an assaythat measures the antioxidant activity of an ingredient or composition.In essence, it can quantify the degree and length of time it takes toinhibit the action of an oxidizing agent such as oxygen radicals thatare known to cause damage cells (e.g., skin cells). The ORAC value ofthe compositions of the present invention can be determined by methodsknown to those of ordinary skill in the art (see U.S. Publication Nos.2004/0109905 and 2005/0163880; Cao et al. (1993)), all of which areincorporated by reference). In summary, the assay described in Cao etal. (1993) measures the ability of antioxidant compounds in testmaterials to inhibit the decline of B-phycoerythrm (B-PE) fluorescencethat is induced by a peroxyl radical generator, AAPH.

D. Compositions of the Present Invention

It is contemplated that the compositions of the present invention caninclude any of the skin actives or any combination thereof describedthroughout this specification. In particular aspects, the skin activescan be combined (e.g., palmitoyl tetrapeptide-7, methylsilanolmannuronate, Lactobacillus ferment, and plant extracts from Punicagranatum, Castanea sativa, Gossypium hirsutum, and Euterpe oleracea).The compositions can include any number of combinations of additionalingredients described throughout this specification. The concentrationsof the any ingredient within the compositions can vary. In non-limitingembodiments, for example, the compositions can comprise, consistingessentially of, or consist of, in their final form, for example, atleast about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%,0.0007%, 0.0008%, 0.0009%, 0.0010%, 0.0011%, 0.0012%, 0.0013%, 0.0014%,0.0015%, 0.0016%, 0.0017%, 0.0018%, 0.0019%, 0.0020%, 0.0021%, 0.0022%,0.0023%, 0.0024%, 0.0025%, 0.0026%, 0.0027%, 0.0028%, 0.0029%, 0.0030%,0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%,0.0039%, 0.0040%, 0.0041%, 0.0042%, 0.0043%, 0.0044%, 0.0045%, 0.0046%,0.0047%, 0.0048%, 0.0049%, 0.0050%, 0.0051%, 0.0052%, 0.0053%, 0.0054%,0.0055%, 0.0056%, 0.0057%, 0.0058%, 0.0059%, 0.0060%, 0.0061%, 0.0062%,0.0063%, 0.0064%, 0.0065%, 0.0066%, 0.0067%, 0.0068%, 0.0069%, 0.0070%,0.0071%, 0.0072%, 0.0073%, 0.0074%, 0.0075%, 0.0076%, 0.0077%, 0.0078%,0.0079%, 0.0080%, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%,0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%, 0.0092%, 0.0093%, 0.0094%,0.0095%, 0.0096%, 0.0097%, 0.0098%, 0.0099%, 0.0100%, 0.0200%, 0.0250%,0.0275%, 0.0300%, 0.0325%, 0.0350%, 0.0375%, 0.0400%, 0.0425%, 0.0450%,0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.0575%, 0.0600%, 0.0625%, 0.0650%,0.0675%, 0.0700%, 0.0725%, 0.0750%, 0.0775%, 0.0800%, 0.0825%, 0.0850%,0.0875%, 0.0900%, 0.0925%, 0.0950%, 0.0975%, 0.1000%, 0.1250%, 0.1500%,0.1750%, 0.2000%, 0.2250%, 0.2500%, 0.2750%, 0.3000%, 0.3250%, 0.3500%,0.3750%, 0.4000%, 0.4250%, 0.4500%, 0.4750%, 0.5000%, 0.5250%, 0.0550%,0.5750%, 0.6000%, 0.6250%, 0.6500%, 0.6750%, 0.7000%, 0.7250%, 0.7500%,0.7750%, 0.8000%, 0.8250%, 0.8500%, 0.8750%, 0.9000%, 0.9250%, 0.9500%,0.9750%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%,2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%,3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2%, 4.3%,4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%,5.6%, 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%,6.8%, 6.9%, 7.0%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.8%, 7.9%,8.0%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9.0%, 9.1%,9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14%,15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%,29%, 30%, 35%, 40%, 45%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or99% or any range derivable therein, of at least one of the ingredientsthat are mentioned throughout the specification and claims. Innon-limiting aspects, the percentage can be calculated by weight orvolume of the total composition. A person of ordinary skill in the artwould understand that the concentrations can vary depending on theaddition, substitution, and/or subtraction of ingredients in a givencomposition.

The disclosed compositions of the present invention may also includevarious antioxidants to retard oxidation of one or more components.Additionally, the prevention of the action of microorganisms can bebrought about by preservatives such as various antibacterial andantifungal agents, including but not limited to parabens (e.g.,methylparabens, propylparabens), chlorobutanol, phenol, sorbic acid,thimerosal or combinations thereof.

E. Vehicles

The compositions of the present invention can be incorporated into alltypes of vehicles. Non-limiting examples of suitable vehicles includeemulsions (e.g., water-in-oil, water-in-oil-in-water, oil-in-water,silicone-in-water, water-in-silicone, oil-in-water-in-oil,oil-in-water-in-silicone emulsions), creams, lotions, solutions (bothaqueous and hydro-alcoholic), anhydrous bases (such as lipsticks andpowders), gels, and ointments or by other method or any combination ofthe forgoing as would be known to one of ordinary skill in the art(Remington's, 1990). Variations and other appropriate vehicles will beapparent to the skilled artisan and are appropriate for use in thepresent invention. In certain aspects, it is important that theconcentrations and combinations of the compounds, ingredients, andagents be selected in such a way that the combinations are chemicallycompatible and do not form complexes which precipitate from the finishedproduct.

It is also contemplated that ingredients identified throughout thisspecification, including but not limited to Hydrolyzed myrtus communis(myrtle) leaf extract, Cucurbita pepo (pumpkin) seed extract, Gossypiumhirsutum (cotton) extract, Castanea sativa (chestnut) seed extract,Euterpe oleracea (acai) fruit extract, Punica granatum (pomegranate)sterols or fruit extract, Terminalia ferdinandiana (kakadu plum) fruitextract, Ferula foetida (ferula) root extract, Myriciaria Dubia (camucamu) fruit extract, Palmitoyl tetrapeptide-3, Monomethylsilanetriolmannuronate (also referred to as methylsilanol mannuronate),Lactobacillus ferment extract, or Alteromonas ferment extract, or anycombinations thereof, can be individually or combinatoriallyencapsulated for delivery to a target area such as skin. Non-limitingexamples of encapsulation techniques include the use of liposomes,vesicles, and/or nanoparticles (e.g., biodegradable andnon-biodegradable colloidal particles comprising polymeric materials inwhich the ingredient is trapped, encapsulated, and/or absorbed—examplesinclude nanospheres and nanocapsules) that can be used as deliveryvehicles to deliver the ingredient to skin (see, e.g., U.S. Pat. No.6,387,398; U.S. Pat. No. 6,203,802; U.S. Pat. No. 5,411,744; Kreuter1998).

F. Cosmetic Products and Articles of Manufacture

The composition of the present invention can also be used in manycosmetic products including, but not limited to, sunscreen products,sunless skin tanning products, hair products, finger nail products,moisturizing creams, skin benefit creams and lotions, softeners, daylotions, gels, ointments, foundations, night creams, lipsticks,cleansers, toners, masks, or other known cosmetic products orapplications. Additionally, the cosmetic products can be formulated asleave-on or rinse-off products. In certain aspects, the compositions ofthe present invention are stand-alone products.

G. Additional Ingredients

In addition to the Hydrolyzed myrtus communis (myrtle) leaf extract,Cucurbita pepo (pumpkin) seed extract, Gossypium hirsutum (cotton)extract, Castanea sativa (chestnut) seed extract, Euterpe oleracea(acai) fruit extract, Punica granatum (pomegranate) sterols or fruitextract, Terminalia ferdinandiana (kakadu plum) fruit extract, Ferulafoetida (ferula) root extract, Myriciaria Dubia (camu camu) fruitextract, Palmitoyl tetrapeptide-3, Monomethylsilanetriol mannuronate(also referred to as methylsilanol mannuronate), Lactobacillus fermentextract, and/or Alteromonas ferment extract ingredients disclosedthroughout this specification, compositions of the present invention caninclude additional ingredients such as cosmetic ingredients andpharmaceutical active ingredients. Non-limiting examples of theseadditional ingredients are described in the following subsections.

1. Cosmetic Ingredients

The CTFA International Cosmetic Ingredient Dictionary and Handbook (2004and 2008) describes a wide variety of non-limiting cosmetic ingredientsthat can be used in the context of the present invention. Examples ofthese ingredient classes include: fragrances (artificial and natural),dyes and color ingredients (e.g., Blue 1, Blue 1 Lake, Red 40, titaniumdioxide, D&C blue no. 4, D&C green no. 5, D&C orange no. 4, D&C red no.17, D&C red no. 33, D&C violet no. 2, D&C yellow no. 10, and D&C yellowno. 11), adsorbents, lubricants, solvents, moisturizers (including,e.g., emollients, humectants, film formers, occlusive agents, and agentsthat affect the natural moisturization mechanisms of the skin),water-repellants, UV absorbers (physical and chemical absorbers such asparaminobenzoic acid (“PABA”) and corresponding PABA derivatives,titanium dioxide, zinc oxide, etc.), essential oils, vitamins (e.g. A,B, C, D, E, and K), trace metals (e.g. zinc, calcium and selenium),anti-irritants (e.g. steroids and non-steroidal anti-inflammatories),botanical extracts (e.g. aloe vera, chamomile, cucumber extract, ginkgobiloba, ginseng, and rosemary), anti-microbial agents, antioxidants(e.g., BHT and tocopherol), chelating agents (e.g., disodium EDTA andtetrasodium EDTA), preservatives (e.g., methylparaben andpropylparaben), pH adjusters (e.g., sodium hydroxide and citric acid),absorbents (e.g., aluminum starch octenylsuccinate, kaolin, corn starch,oat starch, cyclodextrin, talc, and zeolite), skin bleaching andlightening agents (e.g., hydroquinone and niacinamide lactate),humectants (e.g., sorbitol, urea, and manitol), exfoliants,waterproofing agents (e.g., magnesium/aluminum hydroxide stearate), skinconditioning agents (e.g., aloe extracts, allantoin, bisabolol,ceramides, dimethicone, hyaluronic acid, and dipotassium glycyrrhizate).Non-limiting examples of some of these ingredients are provided in thefollowing subsections.

a. UV Absorption Agents

UV absorption agents that can be used in combination with thecompositions of the present invention include chemical and physicalsunblocks. Non-limiting examples of chemical sunblocks that can be usedinclude para-aminobenzoic acid (PABA), PABA esters (glyceryl PABA,amyldimethyl PABA and octyldimethyl PABA), butyl PABA, ethyl PABA, ethyldihydroxypropyl PABA, benzophenones (oxybenzone, sulisobenzone,benzophenone, and benzophenone-1 through 12), cinnamates (octylmethoxycinnamate, isoamyl p-methoxycinnamate, octylmethoxy cinnamate,cinoxate, diisopropyl methyl cinnamate, DEA-methoxycinnamate, ethyldiisopropylcinnamate, glyceryl octanoate dimethoxycinnamate and ethylmethoxycinnamate), cinnamate esters, salicylates (homomethyl salicylate,benzyl salicylate, glycol salicylate, isopropylbenzyl salicylate, etc.),anthranilates, ethyl urocanate, homosalate, octisalate, dibenzoylmethanederivatives (e.g., avobenzone), octocrylene, octyl triazone, digalloytrioleate, glyceryl aminobenzoate, lawsone with dihydroxyacetone,ethylhexyl triazone, dioctyl butamido triazone, benzylidene malonatepolysiloxane, terephthalylidene dicamphor sulfonic acid, disodium phenyldibenzimidazole tetrasulfonate, diethylamino hydroxybenzoyl hexylbenzoate, bis diethylamino hydroxybenzoyl benzoate, bisbenzoxazoylphenyl ethylhexylimino triazine, drometrizole trisiloxane,methylene bis-benzotriazolyl tetramethylbutylphenol, andbis-ethylhexyloxyphenol methoxyphenyltriazine,4-methylbenzylidenecamphor, and isopentyl 4-methoxycinnamate.Non-limiting examples of physical sunblocks include, kaolin, talc,petrolatum and metal oxides (e.g., titanium dioxide and zinc oxide).

b. Moisturizing Agents

Non-limiting examples of moisturizing agents that can be used with thecompositions of the present invention include amino acids, chondroitinsulfate, diglycerin, erythritol, fructose, glucose, glycerin, glycerolpolymers, glycol, 1,2,6-hexanetriol, honey, hyaluronic acid,hydrogenated honey, hydrogenated starch hydrolysate, inositol, lactitol,maltitol, maltose, mannitol, natural moisturizing factor, PEG-15butanediol, polyglyceryl sorbitol, salts of pyrollidone carboxylic acid,potassium PCA, propylene glycol, sodium glucuronate, sodium PCA,sorbitol, sucrose, trehalose, urea, and xylitol.

Other examples include acetylated lanolin, acetylated lanolin alcohol,alanine, algae extract, aloe barbadensis, aloe-barbadensis extract, aloebarbadensis gel, althea officinalis extract, apricot (prunus armeniaca)kernel oil, arginine, arginine aspartate, arnica montana extract,aspartic acid, avocado (persea gratissima) oil, barrier sphingolipids,butyl alcohol, beeswax, behenyl alcohol, beta-sitosterol, birch (betulaalba) bark extract, borage (borago officinalis) extract, butcherbroom(ruscus aculeatus) extract, butylene glycol, calendula officinalisextract, calendula officinalis oil, candelilla (euphorbia cerifera) wax,canola oil, caprylic/capric triglyceride, cardamon (elettariacardamomum) oil, carnauba (copernicia cerifera) wax, carrot (daucuscarota sativa) oil, castor (ricinus communis) oil, ceramides, ceresin,ceteareth-5, ceteareth-12, ceteareth-20, cetearyl octanoate, ceteth-20,ceteth-24, cetyl acetate, cetyl octanoate, cetyl palmitate, chamomile(anthemis nobilis) oil, cholesterol, cholesterol esters, cholesterylhydroxystearate, citric acid, clary (salvia sclarea) oil, cocoa(theobroma cacao) butter, coco-caprylate/caprate, coconut (cocosnucifera) oil, collagen, collagen amino acids, corn (zea mays) oil,fatty acids, decyl oleate, dimethicone copolyol, dimethiconol, dioctyladipate, dioctyl succinate, dipentaerythrityl hexacaprylate/hexacaprate,DNA, erythritol, ethoxydiglycol, ethyl linoleate, eucalyptus globulusoil, evening primrose (oenothera biennis) oil, fatty acids, geraniummaculatum oil, glucosamine, glucose glutamate, glutamic acid,glycereth-26, glycerin, glycerol, glyceryl distearate, glycerylhydroxystearate, glyceryl laurate, glyceryl linoleate, glycerylmyristate, glyceryl oleate, glyceryl stearate, glyceryl stearate SE,glycine, glycol stearate, glycol stearate SE, glycosaminoglycans, grape(vitis vinifera) seed oil, hazel (corylus americana) nut oil, hazel(corylus avellana) nut oil, hexylene glycol, hyaluronic acid, hybridsafflower (carthamus tinctorius) oil, hydrogenated castor oil,hydrogenated coco-glycerides, hydrogenated coconut oil, hydrogenatedlanolin, hydrogenated lecithin, hydrogenated palm glyceride,hydrogenated palm kernel oil, hydrogenated soybean oil, hydrogenatedtallow glyceride, hydrogenated vegetable oil, hydrolyzed collagen,hydrolyzed elastin, hydrolyzed glycosaminoglycans, hydrolyzed keratin,hydrolyzed soy protein, hydroxylated lanolin, hydroxyproline, isocetylstearate, isocetyl stearoyl stearate, isodecyl oleate, isopropylisostearate, isopropyl lanolate, isopropyl myristate, isopropylpalmitate, isopropyl stearate, isostearamide DEA, isostearic acid,isostearyl lactate, isostearyl neopentanoate, jasmine (jasminumofficinale) oil, jojoba (buxus chinensis) oil, kelp, kukui (aleuritesmoluccana) nut oil, lactamide MEA, laneth-16, laneth-10 acetate,lanolin, lanolin acid, lanolin alcohol, lanolin oil, lanolin wax,lavender (lavandula angustifolia) oil, lecithin, lemon (citrus medicalimonum) oil, linoleic acid, linolenic acid, macadamia ternifolia nutoil, maltitol, matricaria (chamomilla recutita) oil, methyl glucosesesquistearate, methylsilanol PCA, mineral oil, mink oil, mortierellaoil, myristyl lactate, myristyl myristate, myristyl propionate,neopentyl glycol dicaprylate/dicaprate, octyldodecanol, octyldodecylmyristate, octyldodecyl stearoyl stearate, octyl hydroxystearate, octylpalmitate, octyl salicylate, octyl stearate, oleic acid, olive (oleaeuropaea) oil, orange (citrus aurantium dulcis) oil, palm (elaeisguineensis) oil, palmitic acid, pantethine, panthenol, panthenyl ethylether, paraffin, PCA, peach (prunus persica) kernel oil, peanut (arachishypogaea) oil, PEG-8 C12-18 ester, PEG-15 cocamine, PEG-150 distearate,PEG-60 glyceryl isostearate, PEG-5 glyceryl stearate, PEG-30 glycerylstearate, PEG-7 hydrogenated castor oil, PEG-40 hydrogenated castor oil,PEG-60 hydrogenated castor oil, PEG-20 methyl glucose sesquistearate,PEG40 sorbitan peroleate, PEG-5 soy sterol, PEG-10 soy sterol, PEG-2stearate, PEG-8 stearate, PEG-20 stearate, PEG-32 stearate, PEG40stearate, PEG-50 stearate, PEG-100 stearate, PEG-150 stearate,pentadecalactone, peppermint (mentha piperita) oil, petrolatum,phospholipids, polyamino sugar condensate, polyglyceryl-3 diisostearate,polyquaternium-24, polysorbate 20, polysorbate 40, polysorbate 60,polysorbate 80, polysorbate 85, potassium myristate, potassiumpalmitate, propylene glycol, propylene glycol dicaprylate/dicaprate,propylene glycol dioctanoate, propylene glycol dipelargonate, propyleneglycol laurate, propylene glycol stearate, propylene glycol stearate SE,PVP, pyridoxine dipalmitate, retinol, retinol palmitate, rice (oryzasativa) bran oil, RNA, rosemary (rosmarinus officinalis) oil, rose oil,safflower (carthamus tinctorius) oil, sage (salvia officinalis) oil,sandalwood (santalum album) oil, serine, serum protein, sesame (sesamumindicum) oil, shea butter (butyrospermum parkii), silk powder, sodiumchondroitin sulfate, sodium hyaluronate, sodium lactate, sodiumpalmitate, sodium PCA, sodium polyglutamate, soluble collagen, sorbitanlaurate, sorbitan oleate, sorbitan palmitate, sorbitan sesquioleate,sorbitan stearate, sorbitol, soybean (glycine soja) oil, sphingolipids,squalane, squalene, stearamide MEA-stearate, stearic acid, stearoxydimethicone, stearoxytrimethylsilane, stearyl alcohol, stearylglycyrrhetinate, stearyl heptanoate, stearyl stearate, sunflower(helianthus annuus) seed oil, sweet almond (prunus amygdalus dulcis)oil, synthetic beeswax, tocopherol, tocopheryl acetate, tocopheryllinoleate, tribehenin, tridecyl neopentanoate, tridecyl stearate,triethanolamine, tristearin, urea, vegetable oil, water, waxes, wheat(triticum vulgare) germ oil, and ylang ylang (cananga odorata) oil.

c. Antioxidants

Non-limiting examples of antioxidants that can be used with thecompositions of the present invention include acetyl cysteine, ascorbicacid polypeptide, ascorbyl dipalmitate, ascorbyl methylsilanolpectinate, ascorbyl palmitate, ascorbyl stearate, BHA, BHT, t-butylhydroquinone, cysteine, cysteine HCl, diamylhydroquinone,di-t-butylhydroquinone, dicetyl thiodipropionate, dioleyl tocopherylmethylsilanol, disodium ascorbyl sulfate, distearyl thiodipropionate,ditridecyl thiodipropionate, dodecyl gallate, erythorbic acid, esters ofascorbic acid, ethyl ferulate, ferulic acid, gallic acid esters,hydroquinone, isooctyl thioglycolate, kojic acid, magnesium ascorbate,magnesium ascorbyl phosphate, methylsilanol ascorbate, natural botanicalanti-oxidants such as green tea or grape seed extracts,nordihydroguaiaretic acid, octyl gallate, phenylthioglycolic acid,potassium ascorbyl tocopheryl phosphate, potassium sulfite, propylgallate, quinones, rosmarinic acid, sodium ascorbate, sodium bisulfite,sodium erythorbate, sodium metabisulfite, sodium sulfite, superoxidedismutase, sodium thioglycolate, sorbityl furfural, thiodiglycol,thiodiglycolamide, thiodiglycolic acid, thioglycolic acid, thiolacticacid, thiosalicylic acid, tocophereth-5, tocophereth-10, tocophereth-12,tocophereth-18, tocophereth-50, tocopherol, tocophersolan, tocopherylacetate, tocopheryl linoleate, tocopheryl nicotinate, tocopherylsuccinate, and tris(nonylphenyl)phosphite.

d. Structuring Agents

In other non-limiting aspects, the compositions of the present inventioncan include a structuring agent. Structuring agent, in certain aspects,assist in providing rheological characteristics to the composition tocontribute to the composition's stability. In other aspects, structuringagents can also function as an emulsifier or surfactant. Non-limitingexamples of structuring agents include stearic acid, palmitic acid,stearyl alcohol, cetyl alcohol, behenyl alcohol, stearic acid, palmiticacid, the polyethylene glycol ether of stearyl alcohol having an averageof about 1 to about 21 ethylene oxide units, the polyethylene glycolether of cetyl alcohol having an average of about 1 to about 5 ethyleneoxide units, and mixtures thereof

e. Emulsifiers

In certain aspects of the present invention, the compositions do notinclude an emulsifier. In other aspects, however, the compositions caninclude one or more emulsifiers. Emulsifiers can reduce the interfacialtension between phases and improve the formulation and stability of anemulsion. The emulsifiers can be nonionic, cationic, anionic, andzwitterionic emulsifiers (See McCutcheon's (1986); U.S. Pat. Nos.5,011,681; 4,421,769; 3,755,560). Non-limiting examples include estersof glycerin, esters of propylene glycol, fatty acid esters ofpolyethylene glycol, fatty acid esters of polypropylene glycol, estersof sorbitol, esters of sorbitan anhydrides, carboxylic acid copolymers,esters and ethers of glucose, ethoxylated ethers, ethoxylated alcohols,alkyl phosphates, polyoxyethylene fatty ether phosphates, fatty acidamides, acyl lactylates, soaps, TEA stearate, DEA oleth-3 phosphate,polyethylene glycol 20 sorbitan monolaurate (polysorbate 20),polyethylene glycol 5 soya sterol, steareth-2, steareth-20, steareth-21,ceteareth-20, PPG-2 methyl glucose ether distearate, ceteth-10,polysorbate 80, cetyl phosphate, potassium cetyl phosphate,diethanolamine cetyl phosphate, polysorbate 60, glyceryl stearate,PEG-100 stearate, and mixtures thereof.

f. Silicone Containing Compounds

In non-limiting aspects, silicone containing compounds include anymember of a family of polymeric products whose molecular backbone ismade up of alternating silicon and oxygen atoms with side groupsattached to the silicon atoms. By varying the —Si—O— chain lengths, sidegroups, and crosslinking, silicones can be synthesized into a widevariety of materials. They can vary in consistency from liquid to gel tosolids.

The silicone containing compounds that can be used in the context of thepresent invention include those described in this specification or thoseknown to a person of ordinary skill in the art. Non-limiting examplesinclude silicone oils (e.g., volatile and non-volatile oils), gels, andsolids. In certain aspects, the silicon containing compounds includes asilicone oils such as a polyorganosiloxane. Non-limiting examples ofpolyorganosiloxanes include dimethicone, cyclomethicone,polysilicone-11, phenyl trimethicone, trimethylsilylamodimethicone,stearoxytrimethylsilane, or mixtures of these and other organosiloxanematerials in any given ratio in order to achieve the desired consistencyand application characteristics depending upon the intended application(e.g., to a particular area such as the skin, hair, or eyes). A“volatile silicone oil” includes a silicone oil have a low heat ofvaporization, i.e. normally less than about 50 cal per gram of siliconeoil. Non-limiting examples of volatile silicone oils include:cyclomethicones such as Dow Corning 344 Fluid, Dow Corning 345 Fluid,Dow Corning 244 Fluid, and Dow Corning 245 Fluid, Volatile Silicon 7207(Union Carbide Corp., Danbury, Conn.); low viscosity dimethicones, i.e.dimethicones having a viscosity of about 50 cst or less (e.g.,dimethicones such as Dow Corning 200-0.5 cst Fluid). The Dow CorningFluids are available from Dow Corning Corporation, Midland, Mich.Cyclomethicone and dimethicone are described in the Third Edition of theCTFA Cosmetic Ingredient Dictionary (incorporated by reference) ascyclic dimethyl polysiloxane compounds and a mixture of fully methylatedlinear siloxane polymers end-blocked with trimethylsiloxy units,respectively. Other non-limiting volatile silicone oils that can be usedin the context of the present invention include those available fromGeneral Electric Co., Silicone Products Div., Waterford, N.Y. and SWSSilicones Div. of Stauffer Chemical Co., Adrian, Mich.

g. Essential Oils

Essential oils include oils derived from herbs, flowers, trees, andother plants. Such oils are typically present as tiny droplets betweenthe plant's cells, and can be extracted by several method known to thoseof skill in the art (e.g., steam distilled, enfleurage (i.e., extractionby using fat), maceration, solvent extraction, or mechanical pressing).When these types of oils are exposed to air they tend to evaporate(i.e., a volatile oil). As a result, many essential oils are colorless,but with age they can oxidize and become darker. Essential oils areinsoluble in water and are soluble in alcohol, ether, fixed oils(vegetal), and other organic solvents. Typical physical characteristicsfound in essential oils include boiling points that vary from about 160°to 240° C. and densities ranging from about 0.759 to about 1.096.

Essential oils typically are named by the plant from which the oil isfound. For example, rose oil or peppermint oil are derived from rose orpeppermint plants, respectively. Non-limiting examples of essential oilsthat can be used in the context of the present invention include sesameoil, macadamia nut oil, tea tree oil, evening primrose oil, Spanish sageoil, Spanish rosemary oil, coriander oil, thyme oil, pimento berriesoil, rose oil, anise oil, balsam oil, bergamot oil, rosewood oil, cedaroil, chamomile oil, sage oil, clary sage oil, clove oil, cypress oil,eucalyptus oil, fennel oil, sea fennel oil, frankincense oil, geraniumoil, ginger oil, grapefruit oil, jasmine oil, juniper oil, lavender oil,lemon oil, lemongrass oil, lime oil, mandarin oil, marjoram oil, myrrhoil, neroli oil, orange oil, patchouli oil, pepper oil, black pepperoil, petitgrain oil, pine oil, rose otto oil, rosemary oil, sandalwoodoil, spearmint oil, spikenard oil, vetiver oil, wintergreen oil, orylang ylang. Other essential oils known to those of skill in the art arealso contemplated as being useful within the context of the presentinvention.

h. Thickening Agents

Thickening agents, including thickener or gelling agents, includesubstances which that can increase the viscosity of a composition.Thickeners includes those that can increase the viscosity of acomposition without substantially modifying the efficacy of the activeingredient within the composition. Thickeners can also increase thestability of the compositions of the present invention. In certainaspects of the present invention, thickeners include hydrogenatedpolyisobutene or trihydroxystearin, or a mixture of both.

Non-limiting examples of additional thickening agents that can be usedin the context of the present invention include carboxylic acidpolymers, crosslinked polyacrylate polymers, polyacrylamide polymers,polysaccharides, and gums. Examples of carboxylic acid polymers includecrosslinked compounds containing one or more monomers derived fromacrylic acid, substituted acrylic acids, and salts and esters of theseacrylic acids and the substituted acrylic acids, wherein thecrosslinking agent contains two or more carbon-carbon double bonds andis derived from a polyhydric alcohol (see U.S. Pat. Nos. 5,087,445;4,509,949; 2,798,053; CTFA International Cosmetic Ingredient Dictionary,Fourth edition, 1991, pp. 12 and 80). Examples of commercially availablecarboxylic acid polymers include carbomers, which are homopolymers ofacrylic acid crosslinked with allyl ethers of sucrose or pentaerytritol(e.g., Carbopol™ 900 series from B.F. Goodrich).

Non-limiting examples of crosslinked polyacrylate polymers includecationic and nonionic polymers. Examples are described in U.S. Pat. Nos.5,100,660; 4,849,484; 4,835,206; 4,628,078; 4,599,379).

Non-limiting examples of polyacrylamide polymers (including nonionicpolyacrylamide polymers including substituted branched or unbranchedpolymers) include polyacrylamide, isoparaffin and laureth-7, multi-blockcopolymers of acrylamides and substituted acrylamides with acrylic acidsand substituted acrylic acids.

Non-limiting examples of polysaccharides include cellulose,carboxymethyl hydroxyethylcellulose, cellulose acetate propionatecarboxylate, hydroxyethylcellulose, hydroxyethyl ethylcellulose,hydroxypropylcellulose, hydroxypropyl methylcellulose, methylhydroxyethylcellulose, microcrystalline cellulose, sodium cellulosesulfate, and mixtures thereof. Another example is an alkyl substitutedcellulose where the hydroxy groups of the cellulose polymer ishydroxyalkylated (preferably hydroxy ethylated or hydroxypropylated) toform a hydroxyalkylated cellulose which is then further modified with aC₁₀-C₃₀ straight chain or branched chain alkyl group through an etherlinkage. Typically these polymers are ethers of C₁₀-C₃₀ straight orbranched chain alcohols with hydroxyalkylcelluloses. Other usefulpolysaccharides include scleroglucans comprising a linear chain of (1-3)linked glucose units with a (1-6) linked glucose every three unit.

Non-limiting examples of gums that can be used with the presentinvention include acacia, agar, algin, alginic acid, ammonium alginate,amylopectin, calcium alginate, calcium carrageenan, carnitine,carrageenan, dextrin, gelatin, gellan gum, guar gum, guarhydroxypropyltrimonium chloride, hectorite, hyaluroinic acid, hydratedsilica, hydroxypropyl chitosan, hydroxypropyl guar, karaya gum, kelp,locust bean gum, natto gum, potassium alginate, potassium carrageenan,propylene glycol alginate, sclerotium gum, sodium carboyxmethyl dextran,sodium carrageenan, tragacanth gum, xanthan gum, and mixtures thereof

i. Preservatives

Non-limiting examples of preservatives that can be used in the contextof the present invention include quaternary ammonium preservatives suchas polyquaternium-1 and benzalkonium halides (e.g., benzalkoniumchloride (“BAC”) and benzalkonium bromide), parabens (e.g.,methylparabens and propylparabens), phenoxyethanol, benzyl alcohol,chlorobutanol, phenol, sorbic acid, thimerosal or combinations thereof

2. Pharmaceutical Ingredients

Pharmaceutical active agents are also contemplated as being useful withthe compositions of the present invention. Non-limiting examples ofpharmaceutical active agents include anti-acne agents, agents used totreat rosacea, analgesics, anesthetics, anorectals, antihistamines,anti-inflammatory agents including non-steroidal anti-inflammatorydrugs, antibiotics, antifungals, antivirals, antimicrobials, anti-canceractives, scabicides, pediculicides, antineoplastics, antiperspirants,antipruritics, antipsoriatic agents, antiseborrheic agents, biologicallyactive proteins and peptides, burn treatment agents, cauterizing agents,depigmenting agents, depilatories, diaper rash treatment agents,enzymes, hair growth stimulants, hair growth retardants including DFMOand its salts and analogs, hemostatics, kerotolytics, canker soretreatment agents, cold sore treatment agents, dental and periodontaltreatment agents, photosensitizing actives, skin protectant/barrieragents, steroids including hormones and corticosteroids, sunburntreatment agents, sunscreens, transdermal actives, nasal actives,vaginal actives, wart treatment agents, wound treatment agents, woundhealing agents, etc.

H. Kits

Kits are also contemplated as being used in certain aspects of thepresent invention. For instance, compositions of the present inventioncan be included in a kit. A kit can include a container. Containers caninclude a bottle, a metal tube, a laminate tube, a plastic tube, adispenser, a pressurized container, a barrier container, a package, acompartment, a lipstick container, a compact container, cosmetic pansthat can hold cosmetic compositions, or other types of containers suchas injection or blow-molded plastic containers into which thedispersions or compositions or desired bottles, dispensers, or packagesare retained. The kit and/or container can include indicia on itssurface. The indicia, for example, can be a word, a phrase, anabbreviation, a picture, or a symbol.

The containers can dispense a pre-determined amount of the composition.In other embodiments, the container can be squeezed (e.g., metal,laminate, or plastic tube) to dispense a desired amount of thecomposition. The composition can be dispensed as a spray, an aerosol, aliquid, a fluid, or a semi-solid. The containers can have spray, pump,or squeeze mechanisms. A kit can also include instructions for employingthe kit components as well the use of any other compositions included inthe container. Instructions can include an explanation of how to apply,use, and maintain the compositions.

EXAMPLES

The following examples are included to demonstrate certain non-limitingaspects of the invention. It should be appreciated by those of skill inthe art that the techniques disclosed in the examples which followrepresent techniques discovered by the inventor to function well in thepractice of the invention. However, those of skill in the art should, inlight of the present disclosure, appreciate that many changes can bemade in the specific embodiments which are disclosed and still obtain alike or similar result without departing from the spirit and scope ofthe invention.

Example 1 Nighttime Complex

A combination of skin active ingredients comprising palmitoyltetrapeptide-7, methylsilanol mannuronate, Lactobacillus ferment, andplant extracts from Punica granatum, Castanea sativa, Gossypiumhirsutum, and Euterpe oleracea was tested to determine its efficacy intoning/firming skin, reducing the appearance of fine lines and wrinkles,and hydrating and moisturizing skin. The inventors discovered that thiscombination of skin actives are chemically compatible and produce amulti-functional product that does not individually require largeamounts of these ingredients. It is thought that these low amountssynergistically work together to create the multi-functional product.These ingredients were placed into dermatologically acceptable vehiclesthat were formulated for normal and dry skin types and for combinationand oil skin types. The compositions are provided in Table 1.

TABLE 1* Ingredient Combo/Oily (%) Normal/Dry (%) Skin Actives**Lactobacillus Ferment 0.9 0.9 Euterpe Oleracea Fruit Extract 0.01 0.01Palmitoyl Tetrapeptide-7 0.001 0.001 Punica Granatum Sterols 1.0 1.0Castanea Sativa Seed Extract 0.05 0.05 Gossypium Hirsutum Extract 0.020.02 Methylsilanol Mannuronate 0.01 0.01 Vehicle Water 72.1 60.8Glycerin 4.3 8.3 Butylene Glycol 9.0 3.0 Glyceryl Stearate 1.2 1.3Caprylic/Capric Triglyceride 4.0 3.0 Hydrogenated Polydecene 1.8 4.0Filler*** q.s q.s *Add vehicle ingredients to beaker and heat to 70-75°C. while mixing. Subsequently, add the skin actives and cool to 30° C.with mixing. **A wide variety of commercial suppliers are available forthese ingredients and are contemplated for use in the present invention.The particular non-limiting suppliers of each ingredient used to obtainthe data for the Table 1 formulations are identified above in the bodyof the specification. ***Filler ingredients can be used to modify thetactile properties of the composition (e.g., viscosity, substantively,texture, color, fragrance, etc.) to achieve a desired result.

The composition formulated for combo and oily skin was a cream withmedium viscosity. The texture was smooth and creamy. The color wasopaque. The scent was neutral or fragrance-free. The after feel wasnon-greasy.

The composition formulated for normal to dry skin was a gel/cream with athin viscosity. The texture was smooth and light. The color was opaque.The scent was neutral or fragrance-free. The after feel was non-greasy.

The composition formulated for combo and oily skin was tested on 66panelists having self-classified combination or oily skin types. Thecomposition formulated for normal or dry skin was tested on 63 panelistshaving self-classified normal or dry skin types. Panelists were femalesaged between 32-65 years old having self-perceived mild to moderate finelines/wrinkles on face. The testing parameters included daily topicalapplication of the composition to the face in the evening and removal inthe morning over a period of 12 weeks. Panelists did not use any otherskin treatment products during the testing period.

At the end of the 12 week period, a significant majority (average of75%) of the panelist population agreed that the composition designed forcombination and oily skin types: hydrated the skin; fortified the skin;toned the skin; restored skin luminosity; visibly reduced the appearanceof fine lines and wrinkles; made the skin feel firmer in areas prone tosagging; lifted the skin from within; recovered/increased elasticity ofthe skin; made the skin appear more youthful; made the skin appearyounger; reduced the appearance of environmental damage to skin; reducedthe signs of premature aging; evened skin tone; reduced the appearanceof expression lines; reduced the appearance of dark spots; andnoticeably contoured the skin.

At the end of the 12 week period, a significant majority (average of81%) of the panelist population agreed that the composition designed fornormal and dry skin types: hydrated the skin; fortified the skin; tonedthe skin; restored skin luminosity; reduced the appearance ofenvironmental damage to skin; recovered/increased skin elasticity;visibly reduced the appearance of fine lines and wrinkles; made the skinlook younger; reduced signs of premature skin aging; made the skin feelfirmer in areas prone to sagging; made the skin appear more youthful;reduced the appearance of expression lines; lifted the skin from within;evened the skin tone; and reduced the appearance of dark spots.

For the combination to oily composition, an average of 93% of thepanelists agreed that the composition: applied easily and evenly toskin; had a smooth and lightweight feel on the skin; absorbed quickly onthe skin; had a non-oily or non-greasy feel; had a rich and luxuriousfeel.

For the normal to dry composition, an average of 91% of the panelistsagreed that the composition: applied easily and evenly to skin; had asmooth and lightweight feel on the skin; absorbed quickly on the skin;had a non-oily or non-greasy feel; had a rich and luxurious feel.

Example 2 Testing Vehicles

In addition to the Table 1 formulations, other non-limiting examples ofcompositions of the present invention are described in Tables 2 and 3.These compositions can be used as vehicles to test the efficacy of theactive ingredients to treat skin.

TABLE 2* Ingredient % Concentration (by weight) Phase A Water q.s. to100 Xanthum gum 0.1 M-paraben 0.15 P-paraben 0.1 Citric acid 0.01 PhaseB Cetyl alcohol 4.0 Glyceryl stearate + PEG 100 4.0 Octyl palmitate 4.0Dimethicone 1.0 Tocopheryl acetate 0.2 Phase C Active Ingredients** 2.0*Sprinkle Xanthum gum in water and mix for 10 min. Subsequently, add allingredients in phase A and heat to 70-75° C. Add all items in phase B toseparate beaker and heat to 70-75° C. Mix phases A and B at 70-75° C.Continue mixing and allow composition to cool to 30° C. Subsequently,add phase C ingredient while mixing. **Any of the active ingredients (orcombination thereof) described in the specification can be used. Forinstance, the active ingredients can include Hydrolyzed myrtus communis(myrtle) leaf extract,Cucurbita pepo (pumpkin) seed extract, Gossypiumhirsutum (cotton) extract, Castanea sativa (chestnut) seed extract,Euterpe oleracea (acai) fruit extract, Punica granatum (pomegranate)sterols or fruit extract, Terminalia ferdinandiana (kakadu plum) fruitextract, Ferula foetida (ferula) root extract, Myriciaria Dubia (camucamu) fruit extract, Palmitoyl tetrapeptide-3, Monomethylsilanetriolmannuronate (also referred to as methylsilanol mannuronate),Lactobacillus ferment extract, or Alteromonas ferment extract, or anycombinations thereof. Although the total amount of active ingredients inthe Table 1 formulation is 2% w/w, it is contemplated that the amount ofactive ingredients can be increased or decreased to achieve a desiredresult, where the water amount can be increased/decreased accordingly(e.g., q.s.).

TABLE 3* Ingredient % Concentration (by weight) Phase A Water q.s. to100 M-paraben 0.2 P-paraben 0.1 Na2 EDTA 0.1 Shea butter 4.5 Petrolatum4.5 Glycerin 4.0 Propylene Glycol 2.0 Finsolve TN 2.0 Phase B Sepigel305 2.0 Phase C Active Ingredient(s)** 2.0 *Add ingredients in phase Ato beaker and heat to 70-75° C. while mixing. Subsequently, add thephase B ingredient with phase A and cool to 30° C. with mixing.Subsequently, add phase C ingredient while mixing. **Any of the activeingredients (or combination thereof) described in the specification canbe used. For instance, the active ingredients can include Hydrolyzedmyrtus communis (myrtle) leaf extract, Cucurbita pepo (pumpkin) seedextract, Gossypium hirsutum (cotton) extract, Castanea sativa (chestnut)seed extract,Euterpe oleracea (acai) fruit extract, Punica granatum(pomegranate) sterols or fruit extract, Terminalia ferdinandiana (kakaduplum) fruit extract, Ferula foetida (ferula) root extract, MyriciariaDubia (camu camu) fruit extract, Palmitoyl tetrapeptide-3,Monomethylsilanetriol mannuronate (also referred to as methylsilanolmannuronate), Lactobacillus ferment extract, or Alteromonas fermentextract, or any combinations thereof. Although the total amount ofactive ingredients in the Table 1 formulation is 2% w/w, it iscontemplated that the amount of active ingredients can be increased ordecreased to achieve a desired result, where the water amount can beincreased/decreased accordingly (e.g., q.s.).

Example 3 Non-Limiting Product Formulations

Additional non-limiting skin product formulations of the presentinvention are described in Tables 4-6. The compositions can be made byany known methods in the art. For instance, simple mixing of theingredients into a container can be used. Further, it is contemplatedthat additional ingredients can be added to the product formulations,listed ingredients can be replaced, and/or listed ingredients can beremoved. The concentration ranges of the ingredients can be modified.

TABLE 4* (Night Moisturizer) Ingredient % Concentration (by weight)Water qs to 100 Glycerin 4.0 Butylene Glycol 8.0 Sodium Polyacrylate 0.5Disodium EDTA 0.05 Caprylic/Capric Triglyceride 4.0 Cyclomethicone 1.0Glyceryl Stearate 1.2 Dimethicone, 200 cs 0.5 Cetearyl Alcohol 0.6C12-22 Alcohols 0.4 C12-20 Alkyl Glucoside 0.1 Preservative qs Actives**0.01-3.0 Additional Ingredients q.s. to 100% *Formulated as anoil-in-water emulsion. Additional ingredients can be added to thisformula. For instance, the formula can include: (i) 2 to 5% by weight ofdenatured alcohol; (ii) 1 to 3% by weight of behenyl alcohol; (iii) 1 to3% by weight of nylon-12; (iv) 1 to 3% by weight of ethylene/acrylicacid copolymer; (v) 0.1 to 2% by weight of glyceryl stearate; (vi) 0.1to 2% by weight of aluminum starch octenylsuccinate; and (vii) acombination of Cucurbita pepo (pumpkin) seed extract, Lactobaccillusferment extract, Terminalia ferdinandiana (kakadu plum) fruit extract,Myriciaria Dubia (camu camu) fruit extract, Hydrolyzed myrtus communis(myrtle) leaf extract, Alteromonas ferment extract, and Ferula foetida(ferula) root extract. **Mixture of Euterpe oleracea (acai) fruitextract and Palmitoyl tetrapeptide-7 (from Sederma (France)-RIGIN ™).

TABLE 5* (Day Lotion w/SPF) Ingredient % Concentration (by weight) Waterqs to 100 Glycerin 4.0 Butylene Glycol 2.0 Disodium EDTA 0.05 AcrylatesCopolymer 2.0 Polyacrylamide 0.6 Glyceryl Stearate and PEG-100 Stearate1.75 Cetyl Alcohol 1.75 C13-14 Isoparaffin 0.30 Laureth-7 .08 CetearylAlcohol 0.65 Ceteth-20 Phosphate 0.45 Dicetyl Phosphate 0.2 Homosalate10.0 Dimethicone, 200 cs. 0.75 Octisalate 5.0 Oxybenzone 4.5 Avobenzone3.0 Preservative qs Actives** 0.01 to 3.0 Additional Ingredients q.s. to100 *Formulated as an oil-in-water emulsion. Additional ingredients canbe added to this formula. For instance, the formula can include: (i) 2to 5% by weight of denatured alcohol; (ii) 1 to 3% by weight of behenylalcohol; (iii) 1 to 3% by weight of ethylene/acrylic acid copolymer;(iv) 0.1 to 2% by weight of glyceryl stearate; (v) 0.1 to 2% by weightof aluminum starch octenylsuccinate; and (vi) a combination of Cucurbitapepo (pumpkin) seed extract,Lactobaccillus ferment extract, Terminaliaferdinandiana (kakadu plum) fruit extract, Myriciaria Dubia (camu camu)fruit extract, Hydrolyzed myrtus communis (myrtle) leaf extract,Alteromonas ferment extract, and Ferula foetida (ferula) root extract.**Mixture of Euterpe oleracea (acai) fruit extract and Palmitoyltetrapeptide-7 (from Sederma (France)-RIGIN ™).

TABLE 6* (Cleanser) Ingredient % Concentration (by weight) Water qs to100 Glycerin 25.0 Cocamidopropyl Betaine 15.0 Potassium Myristate 7.0Stearic Acid 1.5 Disodium EDTA 0.1 Triethanolamine 0.25 Preservative qsActives** 0.01 to 3.0 Additional Ingredients q.s. to 100 *Formulated asa soap. Additional ingredients can be added to this formula. Forinstance, the formula can include: (i) 2 to 5% by weight of potassiumlaurate; (ii) 2 to 5% by weight of myristic acid; (iii) 2 to 5% byweight of sodium myristoyl glutamate; (iv) 1 to 3% by weight of PEG-32;(v) 1 to 3% by weight of sorbitol; (vi) 1 to 3% by weight of glycerylstearate; and (vii) a combination of Terminalia ferdinandiana (kakaduplum) fruit extract, Myriciaria Dubia (camu camu) fruit extract, andHydrolyzed myrtus communis (myrtle) leaf extract. **Mixture of Euterpeoleracea (acai) fruit extract and Palmitoyl tetrapeptide-7 (from Sederma(France)-RIGIN ™).

Example 4 Non-Limiting Delivery Vehicles

A non-limiting delivery vehicle for skin active ingredients disclosedthroughout the specification is described in Tables 7-8. The deliveryvehicle can be made by any known methods in the art. For instance,simple mixing of the ingredients into a container can be used. Further,it is contemplated that additional ingredients can be added to thevehicle, listed ingredients can be replaced, and/or listed ingredientscan be removed. The concentration ranges of the ingredients can bemodified as needed for a given product formulation.

TABLE 7 (Delivery Vehicle) Ingredient % Concentration (by weight) Waterq.s. to 100 Glycerin 3 to 30 Butylene Glycol 0.001 to 5 Propylene Glycol0.1 to 2 Phenoxyethanol 0.00001 to 1 Disodium EDTA 0.01 to 1 Actives*0.001 to 5 Additional Ingredients** q.s. to 100 *Any of the skin activeingredients disclosed throughout the specification, or any combinationthereof, can be incorporated into this delivery vehicle. Examples of theactive ingredients include Hydrolyzed myrtus communis (myrtle) leafextract, Cucurbita pepo (pumpkin) seed extract, Gossypium hirsutum(cotton) extract, Castanea sativa (chestnut) seed extract, Euterpeoleracea (acai) fruit extract, Punica granatum (pomegranate) sterols orfruit extract, Terminalia ferdinandiana (kakadu plum) fruit extract,Ferula foetida (ferula) root extract, Myriciaria Dubia (camu camu) fruitextract, Palmitoyl tetrapeptide-3, Monomethylsilanetriol mannuronate(also referred to as methylsilanol mannuronate), Lactobacillus fermentextract, and/or Alteromonas ferment extract, or any combinationsthereof. In particular embodiments, the skin active ingredients includepalmitoyl tetrapeptide, Euterpe oleracea (acai) fruit extract, orTerminalia ferdinandiana (kakadu plum) extract, or any combination ofsuch ingredients. **Any of the additional ingredients disclosedthroughout the specification, or any combination thereof, can beincorporated into this delivery vehicle.

TABLE 8 (Delivery Vehicle) Ingredient % Concentration (by weight) Waterq.s. to 100 Glycerin 3 to 30 Butylene Glycol 0.001 to 5 Propylene Glycol0.1 to 2 Phenoxyethanol 0.00001 to 1 Disodium EDTA 0.01 to 1 Steareth-200.0001 to 0.1 Chlorhexidine Diglunonate 0.00001 to 0.1 Potassium Sorbate0.000001 to 0.1 Preservative (mixture of parabens) 0.001 to 2 Actives*0.001 to 5 Additional Ingredients** q.s. to 100 *Any of the skin activeingredients disclosed throughout the specification, or any combinationthereof, can be incorporated into this delivery vehicle. Examples of theactive ingredients include Hydrolyzed myrtus communis (myrtle) leafextract, Cucurbita pepo (pumpkin) seed extract, Gossypium hirsutum(cotton) extract, Castanea sativa (chestnut) seed extract, Euterpeoleracea (acai) fruit extract, Punica granatum (pomegranate) sterols orfruit extract, Terminalia ferdinandiana (kakadu plum) fruit extract,Ferula foetida (ferula) root extract, Myriciaria Dubia (camu camu) fruitextract, Palmitoyl tetrapeptide-3, Monomethylsilanetriol mannuronate(also referred to as methylsilanol mannuronate), Lactobacillus fermentextract, and/or Alteromonas ferment extract, or any combinationsthereof. In particular embodiments, the skin active ingredients includepalmitoyl tetrapeptide, Euterpe oleracea (acai) fruit extract, orTerminalia ferdinandiana (kakadu plum) extract, or any combination ofsuch ingredients. **Any of the additional ingredients disclosedthroughout the specification, or any combination thereof, can beincorporated into this delivery vehicle.

Example 5 Supporting Data

The Table 4 moisturizer has been shown to moisturize skin, which isuseful in treating a wide range of skin-related conditions (e.g., dryskin, wrinkled skin, aged skin, etc.) (data not included). The Table 5lotion has also been shown to moisturize skin while also protecting theskin from UV radiation (data not included). The Table 6 formulation hasbeen shown to cleanses skin, which is useful in removing dirt, oil,make-up, and other unwanted substances from skin (data not shown). TheTables 7-8 formulations have been shown to be effective vehicles for theskin active ingredients identified throughout the specification and foruse in the Tables 4-6 formulations (data not shown). Each of the Tables4-8 formulations have also been shown to have excellent tactileproperties (i.e., they are “cosmetically elegant”).

Example 4 Assays that can be Used to Test Compositions

The efficacy of any one of the compositions disclosed throughout thespecification and claims (including, for example, the formulationsidentified in Tables 1-8), can be determined by methods known to thoseof ordinary skill in the art. The following are non-limiting assays thatcan be used in the context of the present invention. It should berecognized that other testing procedures can be used, including, forexample, objective and subjective procedures.

Erythema Assay:

An assay to measure the reduction of skin redness can be evaluated usinga Minolta Chromometer. Skin erythema may be induced by applying a 0.2%solution of sodium dodecyl sulfate on the forearm of a subject. The areais protected by an occlusive patch for 24 hrs. After 24 hrs, the patchis removed and the irritation-induced redness can be assessed using thea* values of the Minolta Chroma Meter. The a* value measures changes inskin color in the red region. Immediately after reading, the area istreated with a composition of the present invention. Repeat measurementsare taken at regular intervals to determine the formula's ability toreduce redness and irritation.

Skin Moisture/Hydration Assay:

Skin moisture/hydration benefits can be measured by using impedancemeasurements with the Nova Dermal Phase Meter. The impedance metermeasures changes in skin moisture content. The outer layer of the skinhas distinct electrical properties. When skin is dry it conductselectricity very poorly. As it becomes more hydrated increasingconductivity results. Consequently, changes in skin impedance (relatedto conductivity) can be used to assess changes in skin hydration. Theunit can be calibrated according to instrument instructions for eachtesting day. A notation of temperature and relative humidity can also bemade. Subjects can be evaluated as follows: prior to measurement theycan equilibrate in a room with defined humidity (e.g., 30-50%) andtemperature (e.g., 68-72° C.). Three separate impedance readings can betaken on each side of the face, recorded, and averaged. The T5 settingcan be used on the impedance meter which averages the impedance valuesof every five seconds application to the face. Changes can be reportedwith statistical variance and significance.

Skin Clarity and Reduction in Freckles and Age Spots Assay:

Skin clarity and the reduction in freckles and age spots can beevaluated using a Minolta Chromometer. Changes in skin color can beassessed to determine irritation potential due to product treatmentusing the a* values of the Minolta Chroma Meter. The a* value measureschanges in skin color in the red region. This is used to determinewhether a composition is inducing irritation. The measurements can bemade on each side of the face and averaged, as left and right facialvalues. Skin clarity can also be measured using the Minolta Meter. Themeasurement is a combination of the a*, b, and L values of the MinoltaMeter and is related to skin brightness, and correlates well with skinsmoothness and hydration. Skin reading is taken as above. In onenon-limiting) aspect, skin clarity can be described as L/C where C ischroma and is defined as (a²+b²)^(1/2).

Skin Dryness, Surface Fine Lines, Skin Smoothness, and Skin Tone Assay:

Skin dryness, surface fine lines, skin smoothness, and skin tone can beevaluated with clinical grading techniques. For example, clinicalgrading of skin dryness can be determined by a five point standardKligman Scale: (0) skin is soft and moist; (1) skin appears normal withno visible dryness; (2) skin feels slightly dry to the touch with novisible flaking; (3) skin feels dry, tough, and has a whitish appearancewith some scaling; and (4) skin feels very dry, rough, and has a whitishappearance with scaling. Evaluations can be made independently by twoclinicians and averaged.

Clinical Grading of Skin Tone Assay:

Clinical grading of skin tone can be performed via a ten point analognumerical scale: (10) even skin of uniform, pinkish brown color. Nodark, erythremic, or scaly patches upon examination with a hand heldmagnifying lens. Microtexture of the skin very uniform upon touch; (7)even skin tone observed without magnification. No scaly areas, butslight discolorations either due to pigmentation or erythema. Nodiscolorations more than 1 cm in diameter; (4) both skin discolorationand uneven texture easily noticeable. Slight scaliness. Skin rough tothe touch in some areas; and (1) uneven skin coloration and texture.Numerous areas of scaliness and discoloration, either hypopigmented,erythremic or dark spots. Large areas of uneven color more than 1 cm indiameter. Evaluations were made independently by two clinicians andaveraged.

Clinical Grading of Skin Smoothness Assay:

Clinical grading of skin smoothness can be analyzed via a ten pointanalog numerical scale: (10) smooth, skin is moist and glistening, noresistance upon dragging finger across surface; (7) somewhat smooth,slight resistance; (4) rough, visibly altered, friction upon rubbing;and (1) rough, flaky, uneven surface. Evaluations were madeindependently by two clinicians and averaged.

Skin Smoothness and Wrinkle Reduction Assay with Methods Disclosed inPackman et al. (1978):

Skin smoothness and wrinkle reduction can also be assessed visually byusing the methods disclosed in Packman et al. (1978). For example, ateach subject visit, the depth, shallowness and the total number ofsuperficial facial lines (SFLs) of each subject can be carefully scoredand recorded. A numerical score was obtained by multiplying a numberfactor times a depth/width/length factor. Scores are obtained for theeye area and mouth area (left and right sides) and added together as thetotal wrinkle score.

Skin Firmness Assay with a Hargens Ballistometer:

Skin firmness can be measured using a Hargens ballistometer, a devicethat evaluates the elasticity and firmness of the skin by dropping asmall body onto the skin and recording its first two rebound peaks. Theballistometry is a small lightweight probe with a relatively blunt tip(4 square mm-contact area) was used. The probe penetrates slightly intothe skin and results in measurements that are dependent upon theproperties of the outer layers of the skin, including the stratumcorneum and outer epidermis and some of the dermal layers.

Skin Softness/Suppleness Assay with a Gas Bearing Electrodynamometer:

Skin softness/suppleness can be evaluated using the Gas BearingElectrodynamometer, an instrument that measures the stress/strainproperties of the skin. The viscoelastic properties of skin correlatewith skin moisturization. Measurements can be obtained on thepredetermined site on the cheek area by attaching the probe to the skinsurface with double-stick tape. A force of approximately 3.5 gm can beapplied parallel to the skin surface and the skin displacement isaccurately measured. Skin suppleness can then be calculated and isexpressed as DSR (Dynamic Spring Rate in gm/mm).

Appearance of Lines and Wrinkles Assay with Replicas:

The appearance of lines and wrinkles on the skin can be evaluated usingreplicas, which is the impression of the skin's surface. Silicone rubberlike material can be used. The replica can be analyzed by imageanalysis. Changes in the visibility of lines and wrinkles can beobjectively quantified via the taking of silicon replicas form thesubjects' face and analyzing the replicas image using a computer imageanalysis system. Replicas can be taken from the eye area and the neckarea, and photographed with a digital camera using a low angle incidencelighting. The digital images can be analyzed with an image processingprogram and the are of the replicas covered by wrinkles or fine lineswas determined.

Surface Contour of the Skin Assay with a Profilometer/Stylus Method:

The surface contour of the skin can be measured by using theprofilometer/Stylus method. This includes either shining a light ordragging a stylus across the replica surface. The vertical displacementof the stylus can be fed into a computer via a distance transducer, andafter scanning a fixed length of replica a cross-sectional analysis ofskin profile can be generated as a two-dimensional curve. This scan canbe repeated any number of times along a fix axis to generate a simulated3-D picture of the skin. Ten random sections of the replicas using thestylus technique can be obtained and combined to generate averagevalues. The values of interest include Ra which is the arithmetic meanof all roughness (height) values computed by integrating the profileheight relative to the mean profile height. Rt which is the maximumvertical distance between the highest peak and lowest trough, and Rzwhich is the mean peak amplitude minus the mean peak height. Values aregiven as a calibrated value in mm. Equipment should be standardizedprior to each use by scanning metal standards of know values. Ra Valuecan be computed by the following equation: R_(a)=Standardize roughness;l_(m)=the traverse (scan) length; and y=the absolute value of thelocation of the profile relative to the mean profile height (x-axis).

MELANODERM™ Assay:

In other non-limiting aspects, the efficacy of the compositions of thepresent invention can be evaluated by using a skin analog, such as, forexample, MELANODERM™. Melanocytes, one of the cells in the skin analog,stain positively when exposed to L-dihydroxyphenyl alanine (L-DOPA), aprecursor of melanin. The skin analog, MELANODERM™, can be treated witha variety of bases containing the compositions and whitening agents ofthe present invention or with the base alone as a control.Alternatively, an untreated sample of the skin analog can be used as acontrol.

ORAC Assay:

Oxygen Radical Absorption (or Absorbance) Capacity (ORAC) of thearomatic skin-active ingredients and compositions can also be assayed bymeasuring the antioxidant activity of such ingredients or compositions.This assay can quantify the degree and length of time it takes toinhibit the action of an oxidizing agent such as oxygen radicals thatare known to cause damage cells (e.g., skin cells). The ORAC value ofthe aromatic skin-active ingredients and compositions can be determinedby methods known to those of ordinary skill in the art (see U.S.Publication Nos. 2004/0109905 and 2005/0163880; Cao et al. (1993)), allof which are incorporated by reference). In summary, the assay describedin Cao et al. (1993) measures the ability of antioxidant compounds intest materials to inhibit the decline of B-phycoerythrm (B-PE)fluorescence that is induced by a peroxyl radical generator, AAPH.

Matrix Metalloproteinase Enzyme Activity (MMP3; MMP9) Assay:

An in vitro matrix metalloprotease (MMP) inhibition assay. MMPs areextracellular proteases that play a role in many normal and diseasestates by virtue of their broad substrate specificity. MMP3 substratesinclude collagens, fibronectins, and laminin; while MMP9 substratesinclude collagen VII, fibronectins and laminin. Using Colorimetric DrugDiscovery kits from BioMol International for MMP3 (AK-400) and MMP-9(AK-410), this assay is designed to measure protease activity of MMPsusing a thiopeptide as a chromogenic substrate(Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5)5,6. The MMP cleavagesite peptide bond is replaced by a thioester bond in the thiopeptide.Hydrolysis of this bond by an MMP produces a sulfhydryl group, whichreacts with DTNB [5,5′-dithiobis(2-nitrobenzoic acid), Ellman's reagent]to form 2-nitro-5-thiobenzoic acid, which can be detected by itsabsorbance at 412 nm (ε=13,600 M-1 cm-1 at pH 6.0 and above 7).

All of the skin-active ingredients, compositions, or methods disclosedand claimed in this specification can be made and executed without undueexperimentation in light of the present disclosure. While theskin-active ingredients, compositions, or methods of this invention havebeen described in terms of particular embodiments, it will be apparentto those of skill in the art that variations may be applied to theskin-active ingredients, compositions, or methods and in the steps or inthe sequence of steps of the method described herein without departingfrom the concept, spirit and scope of the invention.

1-9. (canceled)
 10. A method of treating skin comprising topicallyapplying to skin in need thereof a composition comprising: (a) palmitoyltetrapeptide 7; and (b) a dermatologically acceptable vehiclecomprising: (i) water; (ii) glycerin; (iii) butylene glycol; and (iv) achelating agent, wherein topical application of the composition to theskin treats the skin, and wherein the composition does not include asunless tanner ingredient.
 11. The method of claim 10, wherein thecomposition is applied to facial skin.
 12. The method of claim 11,wherein the composition evens skin tone.
 13. The method of claim 12,wherein the composition further comprises a skin lightening agent. 14.The method of claim 13, wherein the composition is applied tohyperpigmented skin.
 15. The method of claim 13, wherein the compositionis applied to an age spot, a skin discoloration, or a freckle.
 16. Themethod of claim 11, wherein the composition moisturizes skin.
 17. Themethod of claim 10, wherein the pH of the composition is about 6 to 9.18. The method of claim 10, further comprising palmitoyl oligopeptide.19. The method of claim 10, wherein the chelating agent is disodiumEDTA.